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22633 Flow Cytometry Immunophenotyping

Flow Cytometry Immunophenotyping
Test Code: FLOW
Synonyms/Keywords
Flow Cytometry, Immunophenotyping, Lymphoma / Leukemia Panel​​
Useful For
Diagnosis and monitoring of patients with leukemia and lymphoma.​
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Body fluid​ Sodium-heparin Green Top Tube (GTT)​ ​**See Collection Processing Instructions**​ ​4 mL ​Dependent on WBC Dependent on WBC​
No​ ​CSF Sodium-heparin Green Top Tube (GTT)​; add equal volume of RPMI or HBSS if available ​**See Collection Processing Instructions**​ 2 mL​ Dependent on WBC​ ​Dependent on WBC
​No ​Tissue ​Container/Tube filled with RPMI or HBSS ​​**See Collection Processing Instructions**
 ​ ​ ​
​No​BloodSodium-heparin Green Top Tube (GTT)​ or EDTA Lavender Top Tube (LTT)​**See Collection Processing Instructions**​1 mL​Dependent on WBC​​Dependent on WBC​
​No​Bone MarrowSodium-heparin Green Top Tube (GTT)​ or EDTA Lavender Top Tube (LTT)​​​**See Collection Processing Instructions** 
​1mL​Dependent on WBC​​Dependent on WBC​
Collection Processing Instructions

Acceptable specimens include: Fresh Tissue (in sterile RPMI-1640, Hank's Balanced Salt Solution, Cytogenetics Transport Media, or sterile saline); or Body Fluids (submit in sodium heparin). If tissue is large, cut into several smaller pieces.  Specimen must arrive in Flow Cytometry within 36 hours of collection. Notify Flow Cytometry (800-222-5835, ext. 16385) of specimen arrival in lab. Cells in specimen must be viable.​

 Blood and Bone Marrow: Notify Flow Cytometry Laboratory (800-222-5835, ext. 16385) before sending the specimen.  Specimen must arrive within 48 hours of collection.  Cells in the specimen must be viable.  

Provide CBC and Differential results and 1-2 (stained or unstained) EDTA slide(s) (preferably from within 1 week of collection of specimen for Flow Cytometry Immunophenotyping).  Markers are selected based upon available clinical history and morphology per pathology protocol. 

**For questions regarding other anticoagulants and/or specimen stability, please call 800-222-5835, ext. 16385**

Specimen Stability Information
Specimen Type Temperature Time
Tissue​ Room temperature​ 48 - 72 hours​
Body fluid​ Room temperature​ 48 - 72 hours​
​CSF ​Room temperature ​48 - 72 hours​
​Blood​Room temperature​48 - 72 hours
Bone Marrow​​​Room temperature​​48 - 72 hours
Rejection Criteria
Fixed specimens
​​​​​Specimens more than 72 hours old​
Frozen or refrigerated​
​**Contact Flow Cytometry f​​or suitability prior to rejectio​n of any specimens.**​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday, days only​ 3 days​ Flow Cytometry​
Test Information

This test was developed and its performance characteristics determined by Marshfield Labs.  It has not been cleared or approved by the US Food and Drug Administration.  This test is used for clinical purposes.  It should not be regarded as investigational or for research.

Markers are selected based upon available clinical history and morphology per pathology protocol.

Reference Range Information
Performing Location Reference Range
Marshfield​ Interpretive report​
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
88184​ ​ ​ Flow cytometry, first marker ​ ​
88185​ ​ ​ Additional marker, each ​ ​
88187​ ​ ​ Interpretation, 2 to 8 markers ​ ​
88188​ ​ ​ Interpretation, 9 to 15 markers ​ ​
88189​ ​ ​ Interpretation, 16 or more markers ​ ​
Classification

This test was developed and its performance characteristics determined by Marshfield Labs.  It has not been cleared or approved by the US Food and Drug Administration.  This test is used for clinical purposes.  It should not be regarded as investigational or for research.

Synonyms/Keywords
Flow Cytometry, Immunophenotyping, Lymphoma / Leukemia Panel​​
Ordering Applications
Ordering Application Description
​Cerner ​Flow Cytometry Immunophenotyping
​​
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Body fluid​ Sodium-heparin Green Top Tube (GTT)​ ​**See Collection Processing Instructions**​ ​4 mL ​Dependent on WBC Dependent on WBC​
No​ ​CSF Sodium-heparin Green Top Tube (GTT)​; add equal volume of RPMI or HBSS if available ​**See Collection Processing Instructions**​ 2 mL​ Dependent on WBC​ ​Dependent on WBC
​No ​Tissue ​Container/Tube filled with RPMI or HBSS ​​**See Collection Processing Instructions**
 ​ ​ ​
​No​BloodSodium-heparin Green Top Tube (GTT)​ or EDTA Lavender Top Tube (LTT)​**See Collection Processing Instructions**​1 mL​Dependent on WBC​​Dependent on WBC​
​No​Bone MarrowSodium-heparin Green Top Tube (GTT)​ or EDTA Lavender Top Tube (LTT)​​​**See Collection Processing Instructions** 
​1mL​Dependent on WBC​​Dependent on WBC​
Collection Processing

Acceptable specimens include: Fresh Tissue (in sterile RPMI-1640, Hank's Balanced Salt Solution, Cytogenetics Transport Media, or sterile saline); or Body Fluids (submit in sodium heparin). If tissue is large, cut into several smaller pieces.  Specimen must arrive in Flow Cytometry within 36 hours of collection. Notify Flow Cytometry (800-222-5835, ext. 16385) of specimen arrival in lab. Cells in specimen must be viable.​

 Blood and Bone Marrow: Notify Flow Cytometry Laboratory (800-222-5835, ext. 16385) before sending the specimen.  Specimen must arrive within 48 hours of collection.  Cells in the specimen must be viable.  

Provide CBC and Differential results and 1-2 (stained or unstained) EDTA slide(s) (preferably from within 1 week of collection of specimen for Flow Cytometry Immunophenotyping).  Markers are selected based upon available clinical history and morphology per pathology protocol. 

**For questions regarding other anticoagulants and/or specimen stability, please call 800-222-5835, ext. 16385**

Specimen Stability Information
Specimen Type Temperature Time
Tissue​ Room temperature​ 48 - 72 hours​
Body fluid​ Room temperature​ 48 - 72 hours​
​CSF ​Room temperature ​48 - 72 hours​
​Blood​Room temperature​48 - 72 hours
Bone Marrow​​​Room temperature​​48 - 72 hours
Rejection Criteria
Fixed specimens
​​​​​Specimens more than 72 hours old​
Frozen or refrigerated​
​**Contact Flow Cytometry f​​or suitability prior to rejectio​n of any specimens.**​
Useful For
Diagnosis and monitoring of patients with leukemia and lymphoma.​
Reference Range Information
Performing Location Reference Range
Marshfield​ Interpretive report​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday, days only​ 3 days​ Flow Cytometry​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
88184​ ​ ​ Flow cytometry, first marker ​ ​
88185​ ​ ​ Additional marker, each ​ ​
88187​ ​ ​ Interpretation, 2 to 8 markers ​ ​
88188​ ​ ​ Interpretation, 9 to 15 markers ​ ​
88189​ ​ ​ Interpretation, 16 or more markers ​ ​
Classification

This test was developed and its performance characteristics determined by Marshfield Labs.  It has not been cleared or approved by the US Food and Drug Administration.  This test is used for clinical purposes.  It should not be regarded as investigational or for research.

For most current information refer to the Marshfield Laboratory online reference manual.