-Citrated Blue Top Tube (BTT) must be drawn to the fill line.
-Completely invert tube three or four times to mix. Do Not Shake.
-Centrifuge tube at 3000 rpm for 10 minutes or Stat Spin for 3 minutes at 8500 rpm with the brake OFF as soon
as possible after collection.
-If testing cannot be performed within specimen stability transfer plasma (avoiding platelet contamination) to
polypropylene tube with plastic pipette for storage within two hours of collection.
-If sending to another testing location: If combined with other coag testing send only one frozen,
plasma aliquot (1mL) for all tests, with specimen label. Also send original Blue Top Tube (BTT) in case sample needs to be checked for clot.