PREFERRED: After brushing sample is obtained and retracted into the sheath, the brush and part of the brush sheath is cut off and placed into normal saline. Add enough saline to cover to the top of the brush and send to lab as soon as possible.
If there will be delay of more than two hours, place brush in 5-10 mL saline to rinse. If possible, gently vortex specimen container with brush inside to dislodge an optimal amount of cellular material. Remove brush and add 30 mL of Cytolyt.