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22509 Culture, Fungus-Dermal

Culture, Fungus-Dermal
Test Code: FSKIN
Synonyms/Keywords
​Dermatophyte culture
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Skin, hair, or nail scrapings​
Sterile, leakproof container 
(e.g. Sage container)​
 ESwab™ or other Amies medium based swab

Several skin flakes or nail clippings​

1 ESwab or 1 Amies medium based swab

 1-2 nails, several skin flakes​ ​
Collection Processing Instructions
Specimens should be delivered to the microbiology laboratory as soon as possible.  Culture of specimens older than 24 hours may lead to a reduction in recovery of microorganisms.  Do not freeze specimens at -20°C, or in a frost-free freezer.
 
Specimens should be collected in sterile leakproof containers without preservatives.  Swab-collected specimens are not recommended.


Swabs:
- ESwab: one swab can be collected for Gram stain, aerobic, anaerobic and fungal cultures.
- Other Amies medium swab types: collect one swab for every culture and stain (including Gram) to be ordered.

See ESwab collection video for more information 

See Fungus, Direct Wet Prep (KOHFS) for direct stain information.
Specimen Stability Information
Specimen Type

 Temperature Time
Swabs (single, dual) in preservative media (Amies)​

​
​Room Temperature
​<= 4 hours
​Refrigerate
​<24 hours
ESwabs ​ Room Temperature​ < 48 hours
​Refrigerate ​< 48 hours
​All other specimen types ​Room Temperature < 72 hours
Rejection Criteria
Dry Swabs
Specimens submitted in formalin or other preservatives​
​Swab specimens collected in preservative media (Amies) > 24 hours
​ESwab specimens >48 hours
​Leaking specimens
​Specimens older than 3​ days
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield Monday through Friday​ ​ Conventional culture, microscopic exam with fungal-specific stain, manual or automated identification methods and nucleic acid sequence analysis as needed.
Test Information

​The fungal load in a clinical specimen may be low. Specimens with less than the recommended volumes may therefore lead to a false negative result.

‘ESwab’ is a trademark of Copan Diagnostics.
Reference Range Information
Performing Location Reference Range
All Performing Sites Culture negative for Fungi​

See Critical Value List for list of current critical values.

Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
87101​ ​ ​ Culture​ ​
87106​ ​ ​ (Yeast) Organism Identification ​as needed
87107​ ​ ​ (Mold) Organism Identification ​as needed
​87153 ​ ​ ​Identification by nucleic acid sequencing method ​as needed
Synonyms/Keywords
​Dermatophyte culture
Ordering Applications
Ordering Application Description
​Centricity ​Cult. Fungus-Dermal
​Cerner ​Fungus Culture-Dermal
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)		 Pediatric Minimum Volume
(no repeat)
No​ Skin, hair, or nail scrapings​
Sterile, leakproof container 
(e.g. Sage container)​
 ESwab™ or other Amies medium based swab

Several skin flakes or nail clippings​

1 ESwab or 1 Amies medium based swab

 1-2 nails, several skin flakes​ ​
Collection Processing
Specimens should be delivered to the microbiology laboratory as soon as possible.  Culture of specimens older than 24 hours may lead to a reduction in recovery of microorganisms.  Do not freeze specimens at -20°C, or in a frost-free freezer.
 
Specimens should be collected in sterile leakproof containers without preservatives.  Swab-collected specimens are not recommended.


Swabs:
- ESwab: one swab can be collected for Gram stain, aerobic, anaerobic and fungal cultures.
- Other Amies medium swab types: collect one swab for every culture and stain (including Gram) to be ordered.

See ESwab collection video for more information 

See Fungus, Direct Wet Prep (KOHFS) for direct stain information.
Specimen Stability Information
Specimen Type

 Temperature Time
Swabs (single, dual) in preservative media (Amies)​

​
​Room Temperature
​<= 4 hours
​Refrigerate
​<24 hours
ESwabs ​ Room Temperature​ < 48 hours
​Refrigerate ​< 48 hours
​All other specimen types ​Room Temperature < 72 hours
Rejection Criteria
Dry Swabs
Specimens submitted in formalin or other preservatives​
​Swab specimens collected in preservative media (Amies) > 24 hours
​ESwab specimens >48 hours
​Leaking specimens
​Specimens older than 3​ days
Reference Range Information
Performing Location Reference Range
All Performing Sites Culture negative for Fungi​

See Critical Value List for list of current critical values.

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield Monday through Friday​ ​ Conventional culture, microscopic exam with fungal-specific stain, manual or automated identification methods and nucleic acid sequence analysis as needed.
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)		 Quantity Description Comments
87101​ ​ ​ Culture​ ​
87106​ ​ ​ (Yeast) Organism Identification ​as needed
87107​ ​ ​ (Mold) Organism Identification ​as needed
​87153 ​ ​ ​Identification by nucleic acid sequencing method ​as needed
For most current information refer to the Marshfield Laboratory online reference manual.