This test includes a smear exam.
Specimens should be delivered to the microbiology laboratory as soon as possible. Culture of specimens older than 24 hours may lead to a reduction in recovery of microorganisms. Do not freeze specimens at -20°C, or in a frost-free freezer.
Specimens should be collected in sterile leakproof containers without preservatives. A sterile anticoagulant such as heparin or SPS may be used in collection tubes to prevent clots in body fluids. Swab-collected specimens and fecal specimens are NOT recommended.
Swabs: - ESwab: collect one swab for Gram stain, aerobic and anaerobic cultures, and an additional swab for each additional culture type or stain (e.g. fungus or mycobacteria). - Other Amies medium swab types: collect three swabs for AF culture and one swab for each additional culture and stain to be ordered.
For sputum, three samples are recommended (one early morning from deep cough or expectorated sputa induced by aerosolization) eight to twelve hours apart. For urine, three specimens, one per day for three consecutive days are recommended. For gastric lavage fluid, three early morning specimens collected on separate days are recommended. Volumes >15 mL will NOT be accepted. For blood and bone marrow mycobacteria culture, see CULTURE, MYCOBACTERIA, BLOOD.
Conventional culture, microscopic exam with mycobacteria-specific stain, standard susceptibility testing, manual or automated identification methods, and nucleic acid sequence analysis as needed
Concentrated/unconcentrated specimen negative for acid-fast bacilli
Culture negative for acid-fast bacilli