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22471 Cortisol, Urinary Free 24 Hour

Cortisol, Urinary Free 24 Hour
Test Code: U-CORT
Synonyms/Keywords
Cortisol, Urinary Free, Urinary Free Cortisol​
Test Components
24 hour Urine Cortisol and Creatinine​
Useful For
Urine Free Cortisol is a sensitive test for the various types of adrenocortical dysfunction, particularly hypercortisolism (Cushing's Syndrome).  This test is the preferred screening test for Cushing's Syndrome.  This test has limited usefulness in the evaluation of adrenal insufficiency.​
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
No​ Urine, 24 Hour​ 24 hr urine jug - no preservative​ 10 mL aliquot from well mixed 24 hr urine​ 5 mL aliquot ​ 2 mL aliquot​
Collection Processing Instructions
No special patient preparation is required, but avoid all forms of stress to the patient, since this may cause elevated Urinary Cortisol levels.  Indicate collection times.  Total volume must be measured, recorded and included with the test request.  Due to diurnal variations in urinary cortisol, collection must be 24 hours +/- 2 hours.  ​
Specimen Stability Information
Specimen Type Temperature Time
Urine​ Refrigerate​ 1 week​
 Freeze​ 1 month​
Rejection Criteria
Urine collected with a preservative
Collection must be 24 hours +/- 2 hours​
Random specimens​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​

Tuesday, Friday​

Samples must be in the performing department
by 8am on the day of testing for results the same day

6-8 hours​ UHPLC MS/MS-Waters TQ-XS System
Test Information
This test was developed and its performance characteristics determined by Marshfield Labs. This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) to perform high complexity testing.
Reference Range Information
Performing Location Reference Range
Marshfield​

0-2 years: Reference range not established

3-8 years: 1.4-20.0 ug/24 hours

9-12 years: 2.6-37.0 ug/24 hours

13-17 years: 4.0-56.0 ug/24 hours

>=18 years: 3.5-45.0 ug/24 hours

Pediatric ranges based on literature review​

Interpretation
Urine Free Cortisol is a sensitive test for the various types of adrenocortical dysfunction, particularly hypercortisolism (Cushing's Syndrome).  This test is the preferred screening test for Cushing's Syndrome.  This test has limited usefulness in the evaluation of adrenal insufficiency.  Pregnancy values may be elevated to twice the upper limit of normal.​
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
82530​
Classification
This test was developed and its performance characteristics determined by Marshfield Labs. This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) to perform high complexity testing.
Synonyms/Keywords
Cortisol, Urinary Free, Urinary Free Cortisol​
Test Components
24 hour Urine Cortisol and Creatinine​
Ordering Applications
Ordering Application Description
​Centricity ​Cortisol, Free-24hr
​Cerner ​Cortisol, Urinary Free, 24 Hour
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
No​ Urine, 24 Hour​ 24 hr urine jug - no preservative​ 10 mL aliquot from well mixed 24 hr urine​ 5 mL aliquot ​ 2 mL aliquot​
Collection Processing
No special patient preparation is required, but avoid all forms of stress to the patient, since this may cause elevated Urinary Cortisol levels.  Indicate collection times.  Total volume must be measured, recorded and included with the test request.  Due to diurnal variations in urinary cortisol, collection must be 24 hours +/- 2 hours.  ​
Specimen Stability Information
Specimen Type Temperature Time
Urine​ Refrigerate​ 1 week​
 Freeze​ 1 month​
Rejection Criteria
Urine collected with a preservative
Collection must be 24 hours +/- 2 hours​
Random specimens​
Useful For
Urine Free Cortisol is a sensitive test for the various types of adrenocortical dysfunction, particularly hypercortisolism (Cushing's Syndrome).  This test is the preferred screening test for Cushing's Syndrome.  This test has limited usefulness in the evaluation of adrenal insufficiency.​
Test Components
24 hour Urine Cortisol and Creatinine​
Reference Range Information
Performing Location Reference Range
Marshfield​

0-2 years: Reference range not established

3-8 years: 1.4-20.0 ug/24 hours

9-12 years: 2.6-37.0 ug/24 hours

13-17 years: 4.0-56.0 ug/24 hours

>=18 years: 3.5-45.0 ug/24 hours

Pediatric ranges based on literature review​

Interpretation
Urine Free Cortisol is a sensitive test for the various types of adrenocortical dysfunction, particularly hypercortisolism (Cushing's Syndrome).  This test is the preferred screening test for Cushing's Syndrome.  This test has limited usefulness in the evaluation of adrenal insufficiency.  Pregnancy values may be elevated to twice the upper limit of normal.​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​

Tuesday, Friday​

Samples must be in the performing department
by 8am on the day of testing for results the same day

6-8 hours​ UHPLC MS/MS-Waters TQ-XS System
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
82530​
Classification
This test was developed and its performance characteristics determined by Marshfield Labs. This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) to perform high complexity testing.
For most current information refer to the Marshfield Laboratory online reference manual.