Quantitative direct agglutination procedure for the detection of antibody to B. abortus, B. melitensis, and B. suis.
Antibodies to Brucella canis, a rare cause of brucellosis, are not detected by this method.
Store serum specimen at 2-8 degrees C and transport with cool pack.
The assay does not detect antibodies to B. canis or other rough types, such as the vaccine strain B. abortus RB51. Serology results should be interpreted in combination with other clinical, laboratory, and epidemiologic findings. Cross-reactions may occur with Francisella tularensis, Vibrio cholerae, Yersinia enterocolitica serotype O:9, Escherichia coli O:15, Afipia clevelandensis, and other antigenically related species.
Brucellosis is often related to occupational exposure. Serological results must be critically assessed along with clinical findings and occupational factors before a diagnosis is made.