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22374 Cytology, Bronchial Brushing

Cytology, Bronchial Brushing
Test Code: Pathology Non-Gyn Request
Synonyms/Keywords
​​​Bronchial Brush for Cytology​
Useful For

​Utilized to aid in the diagnosis and localization of lung tumors.

Samples are obtained by passing a bronchial brush through a bronchoscope to collect cellular samples that may be missed with the bronchial wash or lavage technique. 

Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Brushing​ Prefilled Wet Prep Vial,
Centrifuge Tube,
Sage Container​​
Collection Processing Instructions
PREFERRED:  After bronchial brushing sampling is completed, the brush and part of the brush sheath is cut off and placed into normal saline.  Add enough saline to cover to the top of the brush and send to lab as soon as possible. 
 
If there will be delay of more than two hours, place brush in 5-10 mL saline to rinse.  If possible, gently vortex specimen container with brush inside to dislodge an optimal amount of cellular material.  Remove brush and add 30 mL of Cytolyt.

NOTE:  Various sites may be sampled using separ​ate brushes.  If multiple sites are sampled, place each brush into a separate specimen container and indicate appropriate site on each container label.

Acceptable Body Sites
Bronchial, Left Upper Lobe (LUL) Bronchial, Left Lower Lobe (LLL) Bronchial, Right Upper Lobe (RUL), Right Lower Lobe (RLL) Bronchial, Right Middle Lobe (RML) Bronchial ​
Specimen Stability Information
Specimen Type Temperature
Brushing​ Refrigerate​
Interference
Specimens not placed in saline or saline/alcohol fixation within two hours of collection may be unacceptable.  Consult Cytopathology with questions (800-222-5835, ext. 1-6155).​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday-Friday​ 2 day​
ThinPrep, Light Microscopy​
Test Information
Include specimen source, pertinent patient history, clinical information and appropriate ICD-10 code on the request form or electronic order.
 
If multiple specimens are collected during a bronchial procedure, each specimen must be accompanied by a separate electronic order or requisition form.  Label each container with appropriate site, date and time of fixation and fixative type.
 
Additional special stains may be requested.  Add the appropriate comment for the requested special stains (i.e. Fungus/Silver Stain/MS or Iron Stain/FE/Hemosiderin) to the electronic order or requisition form.  ​
Reference Range Information
Performing Location Reference Range
Marshfield​ Interpretative Report​
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
88112​ Cytology ThinPrep​
88312​ Special Stain​

​88313




​​Special Stain
Synonyms/Keywords
​​​Bronchial Brush for Cytology​
Ordering Applications
Ordering Application Description

​Cerner
​​Pathology Non-Gyn Request
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Brushing​ Prefilled Wet Prep Vial,
Centrifuge Tube,
Sage Container​​
Collection Processing
PREFERRED:  After bronchial brushing sampling is completed, the brush and part of the brush sheath is cut off and placed into normal saline.  Add enough saline to cover to the top of the brush and send to lab as soon as possible. 
 
If there will be delay of more than two hours, place brush in 5-10 mL saline to rinse.  If possible, gently vortex specimen container with brush inside to dislodge an optimal amount of cellular material.  Remove brush and add 30 mL of Cytolyt.

NOTE:  Various sites may be sampled using separ​ate brushes.  If multiple sites are sampled, place each brush into a separate specimen container and indicate appropriate site on each container label.

Acceptable Body Sites
Bronchial, Left Upper Lobe (LUL) Bronchial, Left Lower Lobe (LLL) Bronchial, Right Upper Lobe (RUL), Right Lower Lobe (RLL) Bronchial, Right Middle Lobe (RML) Bronchial ​
Specimen Stability Information
Specimen Type Temperature
Brushing​ Refrigerate​
Interference
Specimens not placed in saline or saline/alcohol fixation within two hours of collection may be unacceptable.  Consult Cytopathology with questions (800-222-5835, ext. 1-6155).​
Useful For

​Utilized to aid in the diagnosis and localization of lung tumors.

Samples are obtained by passing a bronchial brush through a bronchoscope to collect cellular samples that may be missed with the bronchial wash or lavage technique. 

Reference Range Information
Performing Location Reference Range
Marshfield​ Interpretative Report​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday-Friday​ 2 day​
ThinPrep, Light Microscopy​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
88112​ Cytology ThinPrep​
88312​ Special Stain​

​88313




​​Special Stain
For most current information refer to the Marshfield Laboratory online reference manual.