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22374 Cytology, Bronchial Brushing

Cytology, Bronchial Brushing
Test Code: CY-NON
Synonyms/Keywords
Bronchial Brush for Cytology​
Useful For

​UItilized to aid in the diagnosis and localization of lung tumors.

Samples are obtained by passing a bronchial brush through a brochoscope to collect cellular samples that may be missed with the bronchial wash or lavage technique. 

Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Brushing​ Prefilled Wet Prep Vial,
Centrifuge Tube,
Sage Container​​
Collection Processing Instructions
PREFERRED:  After bronchial brushing sampling is completed, the brush and part of the brush sheath is cut off and placed into normal saline.  Add enough saline to cover to the top of the brush and send to lab as soon as possible. 
 
If there will be delay of more than two hours, place brush in 5-10 mL saline to rinse.  If possible, gently vortex specimen container with brush inside to dislodge an optimal amount of cellular material.  Remove brush and add 30 mL of Cytolyt or an equal volume of 50% ethyl alcohol to the saline rinse.
 
ALTERNATE:  Prepare 1-2 smears on frosted slides from the brush(es) before placing the brushings in 5-10 mL saline.  Immediately fix the smears with spray fixative or immerse in 95% ethyl alcohol.  Gently vortex saline container with brushes inside.  Remove brush and add 30 mL of Cytolyt or an equal volume of 50% ethyl alcohol to the saline rinse.  Send both the fixed smears and saline-fixed rinse.
 
NOTE:  Various sites may be sampled using separate brushes.  If multiple sites are sampled, place each brush into a separate specimen container and indicate appropriate site on each container label.
 
NOTE:  If a culture is also requested on the same specimen, collect specimen in a sage container without the alcohol fixative (fresh specimen).  Specimen must be sent to Microbiology for culturing BEFORE adding the alcohol fixative.​
Acceptable Body Sites
Bronchial, Left Upper Lobe (LUL) Bronchial, Left Lower Lobe (LLL) Bronchial, Right Upper Lobe (RUL), Right Lower Lobe (RLL) Bronchial, Right Middle Lobe (RML) Bronchial ​
Specimen Stability Information
Specimen Type Temperature
Brushing​ Refrigerate​
Interference
Specimens not placed in saline or saline/alcohol fixation within two hours of collection may be unacceptable.  Consult Cytopathology with questions (800-222-5835, ext. 1-6155).​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday-Friday​ 1 day​ ThinPrep, Light Microscopy​
Test Information
Include specimen source, pertinent patient history and clinical information and appropriate ICD-9 code on the electronic order or test requisition form.
 
If multiple specimens are collected during a bronchial procedure, only one Cytology requisition/electronic order needs to be filled out or created.  Label each container with appropriate site, date and time of fixation and fixative type.
 
Additional special stains may be requested.  Add the appropriate comment for the requested special stains (i.e. Fungus/Silver Stain/MS or Iron Stain/FE/Hemosiderin) to the electronic order comments section or write on the test requisition form.​
Reference Range Information
Performing Location Reference Range
Marshfield​ Interpretative Report​
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
88104​ Cytology Smear​
88112​ Cytology ThinPrep​
88312​ Special Stain​
Synonyms/Keywords
Bronchial Brush for Cytology​
Ordering Applications
Ordering Application Description
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Brushing​ Prefilled Wet Prep Vial,
Centrifuge Tube,
Sage Container​​
Collection Processing
PREFERRED:  After bronchial brushing sampling is completed, the brush and part of the brush sheath is cut off and placed into normal saline.  Add enough saline to cover to the top of the brush and send to lab as soon as possible. 
 
If there will be delay of more than two hours, place brush in 5-10 mL saline to rinse.  If possible, gently vortex specimen container with brush inside to dislodge an optimal amount of cellular material.  Remove brush and add 30 mL of Cytolyt or an equal volume of 50% ethyl alcohol to the saline rinse.
 
ALTERNATE:  Prepare 1-2 smears on frosted slides from the brush(es) before placing the brushings in 5-10 mL saline.  Immediately fix the smears with spray fixative or immerse in 95% ethyl alcohol.  Gently vortex saline container with brushes inside.  Remove brush and add 30 mL of Cytolyt or an equal volume of 50% ethyl alcohol to the saline rinse.  Send both the fixed smears and saline-fixed rinse.
 
NOTE:  Various sites may be sampled using separate brushes.  If multiple sites are sampled, place each brush into a separate specimen container and indicate appropriate site on each container label.
 
NOTE:  If a culture is also requested on the same specimen, collect specimen in a sage container without the alcohol fixative (fresh specimen).  Specimen must be sent to Microbiology for culturing BEFORE adding the alcohol fixative.​
Acceptable Body Sites
Bronchial, Left Upper Lobe (LUL) Bronchial, Left Lower Lobe (LLL) Bronchial, Right Upper Lobe (RUL), Right Lower Lobe (RLL) Bronchial, Right Middle Lobe (RML) Bronchial ​
Specimen Stability Information
Specimen Type Temperature
Brushing​ Refrigerate​
Interference
Specimens not placed in saline or saline/alcohol fixation within two hours of collection may be unacceptable.  Consult Cytopathology with questions (800-222-5835, ext. 1-6155).​
Useful For

​UItilized to aid in the diagnosis and localization of lung tumors.

Samples are obtained by passing a bronchial brush through a brochoscope to collect cellular samples that may be missed with the bronchial wash or lavage technique. 

Reference Range Information
Performing Location Reference Range
Marshfield​ Interpretative Report​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday-Friday​ 1 day​ ThinPrep, Light Microscopy​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
88104​ Cytology Smear​
88112​ Cytology ThinPrep​
88312​ Special Stain​
For most current information refer to the Marshfield Laboratory online reference manual.