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22350 Culture, Organism ID, Aerobic Bacteria

Culture, Organism ID, Aerobic Bacteria
Test Code: IDEN
Synonyms/Keywords
​Organism ID, Aerobic Bacteria
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Pure culture of aerobic bacterium​ Sealed agar plates​

 

Agar slant (e.g. chocolate, tryptic soy)

 

Broth (e.g. tryptic soy, thioglycollate)

Collection Processing Instructions
Submit pure culture of organism on agar plates to keep turn-around-time of identification to a minimum.
 
Organisms submitted on an agar slant or in an acceptable broth media will require subculture prior to identification.
 
Do not send primary plates or plates with mixed cultures.  Multiple organisms submitted will be identified and  charged separately. Delay in identification will occur.
 
Ensure that plates are properly sealed (e.g. tape or parafilm) to prevent contamination and exposure to laboratory personnel.  
Specimen Stability Information
Specimen Type Temperature Time
​Agar Plate or Slant After incubation, store at room temperature​ 5 days​
Broth​ Room temperature​ 7 days​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield Monday through Sunday​ 3 days​ Conventional culture, standard susceptibility testing, manual or automated identification methods​
Test Information
If susceptibility testing is desired, please indicate on the test request form, including specific antibiotics to be tested. If not indicated, standard panels will be tested. Susceptibility testing is performed at additional charge. Methods used include disk diffusion, minimum inhibitory concentration (MIC) by broth microdilution, or MIC by Etest®.
 
A listing of the tested and reported antimicrobial agents can be seen at Antimicrobial Susceptibility Panels.Cumulative Antibiograms
 
The microbiology laboratory uses selective reporting of susceptibility results in order to promote rational antimicrobial usage. For more information on selective reporting, please contact the microbiology technical manager. 
 
Standardized AST is not available for all bacterial species, in which case an MIC determined by a non-standardized method and without interpretation may be available upon request. 
 
The microbiology laboratory publishes an annual cumulative antibiogram for commonly isolated bacteria, available at Cumulative Antibiogram Reports.   Printed copies are also available from the microbiology laboratory. ​
 
‘E-test’ is a registered trademark of bioMerieux Inc.
Reference Range Information
Performing Location Reference Range
​Marshfield Interpretive report​
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
87077​
​87184

​KB disc, as needed

​87186 ​MIC, as needed
87153​ Identification by nucleic acid sequencing method, as needed
​87147 ​Sero-grouping, as needed
​87185 ​Enzyme detection, as needed
​87181 ​E-test, gradient strip, as needed
Synonyms/Keywords
​Organism ID, Aerobic Bacteria
Ordering Applications
Ordering Application Description
​Centricity ​None
​Cerner Organism ID​
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Pure culture of aerobic bacterium​ Sealed agar plates​

 

Agar slant (e.g. chocolate, tryptic soy)

 

Broth (e.g. tryptic soy, thioglycollate)

Collection Processing
Submit pure culture of organism on agar plates to keep turn-around-time of identification to a minimum.
 
Organisms submitted on an agar slant or in an acceptable broth media will require subculture prior to identification.
 
Do not send primary plates or plates with mixed cultures.  Multiple organisms submitted will be identified and  charged separately. Delay in identification will occur.
 
Ensure that plates are properly sealed (e.g. tape or parafilm) to prevent contamination and exposure to laboratory personnel.  
Specimen Stability Information
Specimen Type Temperature Time
​Agar Plate or Slant After incubation, store at room temperature​ 5 days​
Broth​ Room temperature​ 7 days​
Reference Range Information
Performing Location Reference Range
​Marshfield Interpretive report​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield Monday through Sunday​ 3 days​ Conventional culture, standard susceptibility testing, manual or automated identification methods​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
87077​
​87184

​KB disc, as needed

​87186 ​MIC, as needed
87153​ Identification by nucleic acid sequencing method, as needed
​87147 ​Sero-grouping, as needed
​87185 ​Enzyme detection, as needed
​87181 ​E-test, gradient strip, as needed
For most current information refer to the Marshfield Laboratory online reference manual.