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22228 Aminolevulinic Acid Dehydratase (ALAD)

Aminolevulinic Acid Dehydratase (ALAD)
Test Code: MISC
Synonyms/Keywords
Porphobilinogen (PBG) Synthase
ALAD (Aminolevulinic Acid Dehydratase) Deficiency Porphyria (ADP)
Doss Porphyria
Useful For

Preferred confirmation test for the diagnosis of aminolevulinic acid dehydratase deficiency porphyria

This test is not useful for detecting lead intoxication. 

Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Whole blood​ Sodium-heparin Green Top Tube (GTT)​ EDTA Lavender Top Tube (LTT) or Lithium-heparin Green Top Tube (GTT)​ Full tube ​4 mL 3 mL​
Collection Processing Instructions

Patient Preparation: Abstinence from alcohol is essential for at least 24 hours prior to specimen collection as ethanol suppresses aminolevulinic acid dehydratase (ALAD) activity, leading to false-positive results.

Collection Instructions: Immediately place specimen on wet ice.

Specimen Stability Information
Specimen Type Temperature Time
​Whole blood ​ Refrigerated​ (preferred) 7 days​
Rejection Criteria

​Gross Hemolyis:  Reject

Interference

​False-positive values may result from enzyme degradation due to improper specimen handling. It is essential to adhere to instructions outlined in the Specimen Required and the Specimen Stability Information fields.

Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Mayo Clinic Laboratories Tuesday, Thursday
2 to 4 days​
Enzymatic End point/Spectrofluorometric​
Reference Lab
Reference Range Information

Reference ranges have not been established for patients who are <16 years of age.

> or =4.0 nmol/L/sec

3.5-3.9 nmol/L/sec (indeterminate)

<3.5 nmol/L/sec (diminished)

Interpretation
Abnormal results are reported with a detailed interpretation including an overview of the results and their significance, a correlation to available clinical information provided with the specimen, differential diagnosis, and recommendations for additional testing when indicated and available.
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​82657
Synonyms/Keywords
Porphobilinogen (PBG) Synthase
ALAD (Aminolevulinic Acid Dehydratase) Deficiency Porphyria (ADP)
Doss Porphyria
Ordering Applications
Ordering Application Description
​COM ​Misc Test
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Whole blood​ Sodium-heparin Green Top Tube (GTT)​ EDTA Lavender Top Tube (LTT) or Lithium-heparin Green Top Tube (GTT)​ Full tube ​4 mL 3 mL​
Collection Processing

Patient Preparation: Abstinence from alcohol is essential for at least 24 hours prior to specimen collection as ethanol suppresses aminolevulinic acid dehydratase (ALAD) activity, leading to false-positive results.

Collection Instructions: Immediately place specimen on wet ice.

Specimen Stability Information
Specimen Type Temperature Time
​Whole blood ​ Refrigerated​ (preferred) 7 days​
Rejection Criteria

​Gross Hemolyis:  Reject

Interference

​False-positive values may result from enzyme degradation due to improper specimen handling. It is essential to adhere to instructions outlined in the Specimen Required and the Specimen Stability Information fields.

Useful For

Preferred confirmation test for the diagnosis of aminolevulinic acid dehydratase deficiency porphyria

This test is not useful for detecting lead intoxication. 

Reference Range Information

Reference ranges have not been established for patients who are <16 years of age.

> or =4.0 nmol/L/sec

3.5-3.9 nmol/L/sec (indeterminate)

<3.5 nmol/L/sec (diminished)

Interpretation
Abnormal results are reported with a detailed interpretation including an overview of the results and their significance, a correlation to available clinical information provided with the specimen, differential diagnosis, and recommendations for additional testing when indicated and available.
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Mayo Clinic Laboratories Tuesday, Thursday
2 to 4 days​
Enzymatic End point/Spectrofluorometric​
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​82657
For most current information refer to the Marshfield Laboratory online reference manual.