Organism Referred for Identification, Aerobic Bacteria (IDENT)

Test Code: MISC

Overview
Ordering
Specimen
Performing
Clinical/Interpretive
Contacts
Coding

Synonyms/Keywords

Organism Referred for Id, Aerobic Bacteria (IDENT), Organism Referred for Identification, Aerobic Bacteria (IDENT), 50012-IDENT

Useful For

Identification of pure isolates of aerobic bacteria

Differentiation of members of the Staphylococcus aureus complex (S aureus, Staphylococcus argenteus, Staphylococcus singaporensis, and Staphylococcus roterodami)

Specimen Requirements

Specimen Type	Preferred Container/Tube	Acceptable Container/Tube	Specimen Volume	Specimen Minimum Volume (allows for 1 repeat)
Bacterial Isolate Swab	E-Swab collection and transport system	Flocked swab and 1-mL liquid Amies transport medium in 12 x 80 mm tube	Entire specimen
Pure culture of organism from source cultured	Agar slant or other appropriate media		Slant of an actively growing bacterial isolate in pure culture

Collection Processing Instructions

Specimen source (anatomical body site) is required.

Isolate description is required including: Gram stain reaction, morphology, and test performed.

Collection Instructions: Bacterial Isolate Swab:

1. Perform isolation of infecting bacteria

2. Utilize the flocked swab to obtain an adequate sample of pure cultured isolate. Do not submit mixed cultures.

3. Place swab into the transport system containing 1-mL liquid Amies transport medium.

4. If needed, break off end of swab and close the transport tube.

5. Place the transport system into the secondary infectious container for shipment.

6. Each isolate must be submitted under a separate order.

Note: For the following organisms, submit an agar slant or other appropriate media to ensure viability upon arrival to the laboratory; Neisseria gonorrhoeae, Campylobacter sp., Helicobacter pylori and any other fastidious organism.

Collection Instructions: Pure culture of organism from source cultured

1. Perform isolation of infecting bacteria.

2. Bacterial organism must be submitted in pure culture, actively growing. Do not submit mixed cultures.

3. Place the agar slant or other appropriate media into the secondary infectious container for shipment.

4. Each isolate must be submitted under a separate order.

Specimen Stability Information

Specimen Type	Temperature
Varies	Ambient (preferred)
Varies	Refrigerated

Rejection Criteria

Agar Plate

Interference

Isolates suspected of being select agent isolates (eg, Bacillus anthracis, Brucella species, Burkholderia mallei, Burkholderia pseudomallei, Francisella tularensis, or Yersinia pestis) should be submitted to client's state health department or the Centers for Disease Control and Prevention for identification confirmation or exclusion.

Performing Laboratory Information

Performing Location	Day(s) Test Performed	Analytical Time	Methodology/Instrumentation
Mayo Clinic Laboratories	Monday through Sunday	5-10 days	Dependent on organism submitted, 1 or more of the following methods will be used: conventional biochemical testing, commercial identification strips or panels, Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) mass spectrometry, and 16S RNA gene sequencing.

Reference Lab

Mayo Clinic Laboratories

Test Information

Organisms are referred to confirm identification or when the identity is unknown. This may provide helpful information regarding the significance of the organism, its role in the disease process, and its possible origin.

Techniques employed may include conventional biochemical analysis, commercial identification strips or panels, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry or sequencing nucleic acid of the 16S ribosomal RNA (rRNA) gene.

When this test is ordered, the reflex tests may be performed at an additional charge. All aerobically growing bacteria submitted will be identified and billed, as appropriate.

Mayo Clinic Laboratories will not perform identification testing on suspected select agents (eg, Bacillus anthracis, Brucella species, Burkholderia mallei, Burkholderia pseudomallei, Francisella tularensis, and Yersinia pestis). Consult with your state health department or the Centers for Disease Control and Prevention regarding identification confirmation or exclusion of such isolates. For more information see www.selectagents.gov/sat/list.htm.

If susceptibility testing is needed; also order ZMMLS / Antimicrobial Susceptibility, Aerobic Bacteria, Varies. If susceptibilities are not appropriate and not performed, ZMMLS will be canceled.

Reference Range Information

Identification of organism

Interpretation

Genus and species are reported on aerobic bacterial isolates, whenever possible.

Bacillus species will be reported out as "Large spore-forming aerobic gram-positive Bacillus, not Bacillus cereus or Bacillus anthracis," unless species identification is specifically requested on the request form.

Outreach CPTs

CPT	Quantity	Description	Comments
87077	1	Organism Referred for Identification, Aerobic Bacteria
87077	1	Identification Commercial Kit	if appropriate
87077	1	Ident by MALDI-TOF mass spec	if appropriate
87077	1	Bacteria Identification	if appropriate
87153	1	Aerobe Ident by Sequencing	if appropriate
87077	1	Additional Identification Procedure	if appropriate
87147	3	Serologic Agglut Method 1 Ident	if appropriate
87147	1	Serologic Agglut Method 2 Ident	if appropriate
87147	4	Serologic Agglut Method 3 Ident	if appropriate
87147	2-6	Serologic Agglut Method 4 Ident	if appropriate
87077	1	Identification Staphylococcus	if appropriate
87077	1	Identification Streptococcus	if appropriate
87798	1	Identification by PCR	if appropriate