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485 E coli Toxin Genome PCR (ECOLITXPCR) w/ Culture (ENTERICULT)

E coli Toxin Genome PCR (ECOLITXPCR) w/ Culture (ENTERICULT)
Test Code: VFECXSO
Synonyms/Keywords
E coli, E. coli PCR, E.col multiplex PCR
Species
Alpaca, Avian, Bovine, Camelid, Canine, Caprine, Equine, Feline, Ferret, Mouse, Other (non-primate), Ovine, Porcine, Rabbit, Rat
Test Category
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Feces
Sterile leakproof container
​Para Pak (C&S)
Cary Blair
​2-10 grams ​2 grams
Collection Processing Instructions
Ship within 2 days of collection.  DO NOT SUBMIT IN GLOVES OR SLEEVES. 
Specimen Stability Information
Specimen Type Temperature Time​
Feces  ​Refrigerate ​3 days
Rejection Criteria
​Frozen or Ambient specimens
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Sent to Wisconsin Veterinary Diagnostic Laboratory (WVDL) Wednesday
Culture:
2-5 days
 
PCR: 24 hours
​Culture on selective media and PCR
Marshfield Center Laboratory Section
Reference Lab Information
Address Telephone Website Link Marshfield Lab Account #
 
445 Easterday Lane
Madison, WI 53706​
​Ph 800-608-8387
Fax 847-574-8085
www.wvdl.wisc.edu ​786
Test Information
​Test detects genes for K99 and F41 fimbrae, heat stable enterotoxin (Sta), intimin, and two Shiga toxins (Stx 1 and 2).
For primate specimens, toxigenic E. coli will be detected as part of a feces culture (VFECE) workup.
Reference Intervals
See Report
Synonyms/Keywords
E coli, E. coli PCR, E.col multiplex PCR
Species
Alpaca, Avian, Bovine, Camelid, Canine, Caprine, Equine, Feline, Ferret, Mouse, Other (non-primate), Ovine, Porcine, Rabbit, Rat
Test Category
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Feces
Sterile leakproof container
​Para Pak (C&S)
Cary Blair
​2-10 grams ​2 grams
Collection Processing Instructions
Ship within 2 days of collection.  DO NOT SUBMIT IN GLOVES OR SLEEVES. 
Specimen Stability Information
Specimen Type Temperature Time​
Feces  ​Refrigerate ​3 days
Rejection Criteria
​Frozen or Ambient specimens
Test Information
​Test detects genes for K99 and F41 fimbrae, heat stable enterotoxin (Sta), intimin, and two Shiga toxins (Stx 1 and 2).
For primate specimens, toxigenic E. coli will be detected as part of a feces culture (VFECE) workup.
Reference Intervals
See Report
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Sent to Wisconsin Veterinary Diagnostic Laboratory (WVDL) Wednesday
Culture:
2-5 days
 
PCR: 24 hours
​Culture on selective media and PCR
Laboratory Section
Reference Lab Information
Address Telephone Website Link Marshfield Lab Account #
 
445 Easterday Lane
Madison, WI 53706​
​Ph 800-608-8387
Fax 847-574-8085
www.wvdl.wisc.edu ​786
For most current information refer to the Marshfield Laboratory online reference manual.