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422 Anaerobic Culture

Anaerobic Culture
Test Code: VANAE
Species
Avian, Bovine, Camelid, Canine, Caprine, Equine, Feline, Ferret, Mouse, Other, Ovine, Porcine, Primate, Rabbit, Rat
Test Category
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Fluid​ ​Liquid Dental Transport ​>0.5 mL ​>0.5 mL
​Small Tissue <5 mm ​A.C.T. 1 Tube ​<5 mm ​<5 mm
​Large Tissue >5 mm ​Sterile, Leak-Proof Container >5 mm ​<5 mm
​Swab ESwab Other anaerobe transport medium ​1 Swab 1 ​Swab
Collection Processing Instructions

To optimize the isolation of clinically significant anaerobic bacteria, extreme care must be taken to avoid contamination with normal flora, to collect the specimen from the active sites of infection, and to maintain anaerobic conditions for the specimen.

Fluid/Aspirate: Submit in Dental transport medium: Cleanse rubber top with alcohol and allow to dry. Inject fluid or aspirate into transport media taking care not to introduce air. Tissue (>5mm): Submit in a screw-capped container and moisten tissue with a few drops of sterile saline to prevent drying. Tissue (<5mm): Place "gently on top" of semi-solid gel in ACT 1 Transport. Do not submerge tissue into semi-solid gel.

Swabs:

  • ESwab: collect one swab for Gram stain, aerobic and anaerobic cultures, and an additional swab for each additional culture type or stain (e.g. fungus or mycobacteria).
  • Other Amies medium swab types: collect one swab for every culture and stain to be ordered.

See eSwab collection video for more information

Swab specimens are not recommended! To optimize recovery of clinically significant anaerobic bacteria, care must be taken to avoid contamination with indigenous flora by collecting the specimen from an active site of infection.

Specimen Stability Information
Specimen Type Temperature Time
Swabs ​Refrigerated ​<= 4 days
All other specimens Room Temperature <= 4 days
Rejection Criteria
Specimens obtained from a source normally colonized with anaerobic flora (e.g. cervix, urethra, vagina, stool ,intestine,and upper respiratory tract)  Note:  Tracheal Washes are unacceptable for anaerobe culture, however transtracheal washes are acceptable because they bypass the upper indigenous respiratory flora
​Specimens not submitted in appropriate anaerobic transport
​Leaking specimens
​Specimens > 4 days old
​​Specimens submitted in Lavender Top Tube (LTT)
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield ​Culture: Monday through Friday ​7 days ​Culture
Marshfield Center Laboratory Section
Test Information

Anaerobic susceptibilities are not routinely performed, although suggestions for treatment are usually provided. Susceptibility testing will be performed only after consultation with veterinarian at an additional cost.

‘ESwab’ is a trademark of Copan Diagnostics.
Reference Intervals
Performing Location Reference Intervals
Marsfhield​ ​No Anaerobes Isolated or No Growth
Species
Avian, Bovine, Camelid, Canine, Caprine, Equine, Feline, Ferret, Mouse, Other, Ovine, Porcine, Primate, Rabbit, Rat
Test Category
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Fluid​ ​Liquid Dental Transport ​>0.5 mL ​>0.5 mL
​Small Tissue <5 mm ​A.C.T. 1 Tube ​<5 mm ​<5 mm
​Large Tissue >5 mm ​Sterile, Leak-Proof Container >5 mm ​<5 mm
​Swab ESwab Other anaerobe transport medium ​1 Swab 1 ​Swab
Collection Processing Instructions

To optimize the isolation of clinically significant anaerobic bacteria, extreme care must be taken to avoid contamination with normal flora, to collect the specimen from the active sites of infection, and to maintain anaerobic conditions for the specimen.

Fluid/Aspirate: Submit in Dental transport medium: Cleanse rubber top with alcohol and allow to dry. Inject fluid or aspirate into transport media taking care not to introduce air. Tissue (>5mm): Submit in a screw-capped container and moisten tissue with a few drops of sterile saline to prevent drying. Tissue (<5mm): Place "gently on top" of semi-solid gel in ACT 1 Transport. Do not submerge tissue into semi-solid gel.

Swabs:

  • ESwab: collect one swab for Gram stain, aerobic and anaerobic cultures, and an additional swab for each additional culture type or stain (e.g. fungus or mycobacteria).
  • Other Amies medium swab types: collect one swab for every culture and stain to be ordered.

See eSwab collection video for more information

Swab specimens are not recommended! To optimize recovery of clinically significant anaerobic bacteria, care must be taken to avoid contamination with indigenous flora by collecting the specimen from an active site of infection.

Specimen Stability Information
Specimen Type Temperature Time
Swabs ​Refrigerated ​<= 4 days
All other specimens Room Temperature <= 4 days
Rejection Criteria
Specimens obtained from a source normally colonized with anaerobic flora (e.g. cervix, urethra, vagina, stool ,intestine,and upper respiratory tract)  Note:  Tracheal Washes are unacceptable for anaerobe culture, however transtracheal washes are acceptable because they bypass the upper indigenous respiratory flora
​Specimens not submitted in appropriate anaerobic transport
​Leaking specimens
​Specimens > 4 days old
​​Specimens submitted in Lavender Top Tube (LTT)
Test Information

Anaerobic susceptibilities are not routinely performed, although suggestions for treatment are usually provided. Susceptibility testing will be performed only after consultation with veterinarian at an additional cost.

‘ESwab’ is a trademark of Copan Diagnostics.
Reference Intervals
Performing Location Reference Intervals
Marsfhield​ ​No Anaerobes Isolated or No Growth
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield ​Culture: Monday through Friday ​7 days ​Culture
Laboratory Section
For most current information refer to the Marshfield Laboratory online reference manual.