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409 Aerobic Culture & Susceptibility

Aerobic Culture & Susceptibility
Test Code: VAEROB
Synonyms/Keywords
​C&S
Test Components
​Culture
Species
Alpaca, Avian, Bovine, Canine, Caprine, Camelid, Equine, Feline, Ferret, Mouse, Other, Ovine, Primate, Porcine, Rabbit, Rat
Test Category
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
​Any ​Sterile, leak-proof container without preservative ESwab™, or other Amies medium based swab ​One Swab
Collection Processing Instructions

Swab specimens should be submitted in ESwab or any commercial swab transport system. ESwabs should arrive in the lab within 4 days of collection for optimal recovery of microbes.

Swabs:

  • ESwab: collect one swab for Gram stain, aerobic and anaerobic cultures, and an additional swab for each additional culture type or stain (e.g. fungus or mycobacteria).
  • Other Amies medium swab types: collect one swab for every culture and stain (including Gram) to be ordered.

See eSwab collection video for more information

Body fluids or tissues should be submitted in a sterile leak-proof container without preservatives. Tissue specimens may require a small amount of non-bacteriostatic saline to prevent drying. Gram stains will routinely be performed on all tissues and fluids, specimen volume permitting.

Specimen Stability Information
Specimen Type Temperature Time
All Specimens ​Refrigerate ​<= 4 days
Rejection Criteria
Tissues in large volume of saline
​Dried swabs or tissues
​Specimens submitted in formalin or Heparin
​Leaking specimens
​Specimens > 4 days old
​Specimens submitted in Lavender Top Tube (LTT)
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield ​Monday through Sunday ​3-4 days ​Culture
Marshfield Center Laboratory Section
Test Information

Up to two susceptibilities will be performed as part of the culture. Each additional susceptibility required will be individually charged. Routine susceptibilities are performed by the micro broth dilution method. MICs will be provided with each susceptibility.

‘ESwab’ is a trademark of Copan Diagnostics.
Reference Intervals
Performing Location Reference Intervals
​Marshfield ​ ​Sterile Sites: No Growth
​Non-Sterile/Mucosal Sites: Mixed Indigenous Flora
Synonyms/Keywords
​C&S
Test Components
​Culture
Species
Alpaca, Avian, Bovine, Canine, Caprine, Camelid, Equine, Feline, Ferret, Mouse, Other, Ovine, Primate, Porcine, Rabbit, Rat
Test Category
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
​Any ​Sterile, leak-proof container without preservative ESwab™, or other Amies medium based swab ​One Swab
Collection Processing Instructions

Swab specimens should be submitted in ESwab or any commercial swab transport system. ESwabs should arrive in the lab within 4 days of collection for optimal recovery of microbes.

Swabs:

  • ESwab: collect one swab for Gram stain, aerobic and anaerobic cultures, and an additional swab for each additional culture type or stain (e.g. fungus or mycobacteria).
  • Other Amies medium swab types: collect one swab for every culture and stain (including Gram) to be ordered.

See eSwab collection video for more information

Body fluids or tissues should be submitted in a sterile leak-proof container without preservatives. Tissue specimens may require a small amount of non-bacteriostatic saline to prevent drying. Gram stains will routinely be performed on all tissues and fluids, specimen volume permitting.

Specimen Stability Information
Specimen Type Temperature Time
All Specimens ​Refrigerate ​<= 4 days
Rejection Criteria
Tissues in large volume of saline
​Dried swabs or tissues
​Specimens submitted in formalin or Heparin
​Leaking specimens
​Specimens > 4 days old
​Specimens submitted in Lavender Top Tube (LTT)
Test Information

Up to two susceptibilities will be performed as part of the culture. Each additional susceptibility required will be individually charged. Routine susceptibilities are performed by the micro broth dilution method. MICs will be provided with each susceptibility.

‘ESwab’ is a trademark of Copan Diagnostics.
Reference Intervals
Performing Location Reference Intervals
​Marshfield ​ ​Sterile Sites: No Growth
​Non-Sterile/Mucosal Sites: Mixed Indigenous Flora
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield ​Monday through Sunday ​3-4 days ​Culture
Laboratory Section
For most current information refer to the Marshfield Laboratory online reference manual.