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# A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
Leptospirosis Antibody IgM
Test Code: VLEPT
Synonyms/Keywords
 Lepto, Leptospirosis, Leptospira
Species
Canine
Test Category
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
​Serum (preferred) ​Red Top Tube (RTT) ​Serum Separator Tube ​0.5 mL ​200 uL
​Plasma ​EDTA Lavender Top Tube (LTT) ​0.5 mL ​200 uL
​Whole Blood ​EDTA Lavender Top Tube (LTT) ​0.5 mL ​200 uL
Collection Processing Instructions
Label tube with animal’s name.  Avoid hemolysis
Specimen Stability Information
Specimen Type Temperature Time
​Serum ​ ​Refrigerate ​1 week
​Frozen ​6 months
​Plasma ​ ​Refrigerate ​1 week
​Frozen ​6 months
​Whole Blood ​Refrigerate ​1 day
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Cleveland ​Monday through Saturday ​Less than 2 hours ​Rapid Immuno Migration
​Marshfield ​Monday through Saturday ​Less than 2 hours ​Rapid Immuno Migration
​Milwaukee ​Monday through Saturday ​Less than 2 hours ​Rapid Immuno Migration
​Minneapolis ​Monday through Saturday ​Less than 2 hours ​Rapid Immuno Migration
Marshfield Center Laboratory Section
Test Information
Tests for antibody to Leptospira serovars Canicola, Pomona, Icterhaemorrhagiae, and Grippotyphosa. Result is reported as Positive or Negative – no individual serovar information will accompany result.
Reference Intervals
Normal result is negative
Interpretation

A positive Leptospira IgM test result indicates exposure to L. canicola, icterohemorrhagica, grippotyphosa or pomona/autumnalis and should be considered supportive of the diagnosis of leptospirosis if the dog has not been recently vaccinated and the clinical signs, history, physical examination findings and other laboratory results support the diagnosis. Ancillary testing (serum chemistry, hematology, urinalysis) and molecular testing (Leptospira PCR in urine or blood) can aid in making a definitive diagnosis of leptospirosis.

A negative IgM test result indicates no exposure to L. canicola, icterohemorrhagica, grippotyphosa or pomona/autumnalis has occurred in the time window of 3 – 10 days up to 6 months prior to testing. Repeat testing for IgM antibodies 3 days following a negative test can confirm exposure status. Negative IgM test results support ruling out leptospirosis provided there is an appropriate time course since exposure. Ancillary testing (serum chemistry, hematology, urinalysis) and molecular testing (Leptospira PCR in urine or blood) can aid in ruling out leptospirosis.

Studies show Leptospira vaccination Ig M antibody wanes in the majority of dogs by 12 weeks and becomes undetectable in all dogs by 26 weeks post vaccination.

Synonyms/Keywords
 Lepto, Leptospirosis, Leptospira
Species
Canine
Test Category
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
​Serum (preferred) ​Red Top Tube (RTT) ​Serum Separator Tube ​0.5 mL ​200 uL
​Plasma ​EDTA Lavender Top Tube (LTT) ​0.5 mL ​200 uL
​Whole Blood ​EDTA Lavender Top Tube (LTT) ​0.5 mL ​200 uL
Collection Processing Instructions
Label tube with animal’s name.  Avoid hemolysis
Specimen Stability Information
Specimen Type Temperature Time
​Serum ​ ​Refrigerate ​1 week
​Frozen ​6 months
​Plasma ​ ​Refrigerate ​1 week
​Frozen ​6 months
​Whole Blood ​Refrigerate ​1 day
Test Information
Tests for antibody to Leptospira serovars Canicola, Pomona, Icterhaemorrhagiae, and Grippotyphosa. Result is reported as Positive or Negative – no individual serovar information will accompany result.
Reference Intervals
Normal result is negative
Interpretation

A positive Leptospira IgM test result indicates exposure to L. canicola, icterohemorrhagica, grippotyphosa or pomona/autumnalis and should be considered supportive of the diagnosis of leptospirosis if the dog has not been recently vaccinated and the clinical signs, history, physical examination findings and other laboratory results support the diagnosis. Ancillary testing (serum chemistry, hematology, urinalysis) and molecular testing (Leptospira PCR in urine or blood) can aid in making a definitive diagnosis of leptospirosis.

A negative IgM test result indicates no exposure to L. canicola, icterohemorrhagica, grippotyphosa or pomona/autumnalis has occurred in the time window of 3 – 10 days up to 6 months prior to testing. Repeat testing for IgM antibodies 3 days following a negative test can confirm exposure status. Negative IgM test results support ruling out leptospirosis provided there is an appropriate time course since exposure. Ancillary testing (serum chemistry, hematology, urinalysis) and molecular testing (Leptospira PCR in urine or blood) can aid in ruling out leptospirosis.

Studies show Leptospira vaccination Ig M antibody wanes in the majority of dogs by 12 weeks and becomes undetectable in all dogs by 26 weeks post vaccination.

Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Cleveland ​Monday through Saturday ​Less than 2 hours ​Rapid Immuno Migration
​Marshfield ​Monday through Saturday ​Less than 2 hours ​Rapid Immuno Migration
​Milwaukee ​Monday through Saturday ​Less than 2 hours ​Rapid Immuno Migration
​Minneapolis ​Monday through Saturday ​Less than 2 hours ​Rapid Immuno Migration
Laboratory Section
For most current information refer to the Marshfield Laboratory online reference manual.