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# A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
TLI, Cobalamin (B12) & Folate
Test Code: VTB12F
Synonyms/Keywords
​TLI, Vitamin B12, EPI (Exocrine Pancreatic Insufficiency)
Test Components
​TLI, Cobalamin, & Folate
Species
Canine
Test Category
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
​Serum ​Red Top Tube (RTT) ​Serum Separator Tube (SST) ​500 uL ​400 uL
Collection Processing Instructions
​Samples should be collected from fasting animals: 12-18 hours for non-diabetic animals; 6 hours for diabetic animals.
 
Pancreatic function must be assessed before these results can be interpreted since they may be abnormal in animals with EPI (Exocrine Pancreatic Insufficiency)
 
Serum/Plasma should be separated from cells within one hour of collection and frozen as soon as possible unless transport to lab is <8 hours.
Acceptable Specimen Types
​Serum
Specimen Stability Information
Specimen Type Temperature Time
​Serum ​Refrigerated ​8 hours
​Frozen (preferred) ​60 days
Rejection Criteria
Plasma
​Room Temperature Samples
Interference
​Helolysis and non-fasting status may interfere with results.
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield ​Monday through Saturday ​2 hours ​Chemiluminescence/Immulite
Marshfield Center Laboratory Section
Test Information
​Reference intervals and interpretative comments from Gastrointestinal Laboratroy - College of Veterinary Medicine Texas A&M University, College Station, TX 77843-4474
Reference Intervals
Performing Location Reference Intervals
​Marshfield ​Cobalamine: 251-908 ng/L
​Folate: 7.7-24.4- ug/L
​TLI: 5.7-45.2 ug/L
Interpretation

cTLI: <5.7 ug/L - Interpretation: Diagnostic for exocrine pancreatic insufficency (EPI)

cTLI: >45.2 ug/L - Interpretation: Increased serum cTLI.  This may be associated with pancreatitis but has also been reported in association with renal falure or emaciation.

Cobalamin: Canine
251-908 – Interpretation: Result within the reference interval
<251, “<150” – Interpretation: Consistent with distal small intestinal disease, EPI or small intestinal bacterial overgrowth.  Check canine TLI to rule out EPI.  Consider oral or parenteral cobalamin supplementation
251-470 – Interpretation: Result is at the low end of the reference interval.  Suggestive of distal small intestinal disease (i.e., ileum), EPI, or dysbiosis.  Check serum canine TLI concentration to rule out EPI. Oral or parenteral supplementation is indicated.
>908, “>1000” – Interpretation: There is no known clinical significance for serum cobalamin concentrations above the upper limit of the reference interval.
 
Folate: Canine
7.7-24.4 – Interpretation: Result is within the reference interval
<7.7, “<1.0” – Interpretation: Decreased serum folate concentration.  Consistent with disease affecting proximal small intestine.  Consider  folate supplementation.
18.8-24.4 – Interpretation: Upper end of the reference interval.  May reflect small intestinal dysbiosis.  In patients with GI signs but without systemic complications (i.e., sever weight loss, hypoalbuminemia, or other) trial therapies with prebiotics (e.g., a diet containing fructooligosaccharieds (FOS)), probiotics, or antibiotics may be considered.  The Gastrointestinal Laboratory at Texas A & M university recommends Tylosin at 25 mg/kg q 12 hrs. PO for 6-8 weeks.
>24.4, “>50.0” – Interpretation: Consistent with small intestinal dysbiosis.  Small intestinal dybiosis often occurs secondary to chronic enteropathy or various other gastrointestinal disorders but can also be primary (idiopathic).
Synonyms/Keywords
​TLI, Vitamin B12, EPI (Exocrine Pancreatic Insufficiency)
Test Components
​TLI, Cobalamin, & Folate
Species
Canine
Test Category
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
​Serum ​Red Top Tube (RTT) ​Serum Separator Tube (SST) ​500 uL ​400 uL
Collection Processing Instructions
​Samples should be collected from fasting animals: 12-18 hours for non-diabetic animals; 6 hours for diabetic animals.
 
Pancreatic function must be assessed before these results can be interpreted since they may be abnormal in animals with EPI (Exocrine Pancreatic Insufficiency)
 
Serum/Plasma should be separated from cells within one hour of collection and frozen as soon as possible unless transport to lab is <8 hours.
Acceptable Specimen Types
​Serum
Specimen Stability Information
Specimen Type Temperature Time
​Serum ​Refrigerated ​8 hours
​Frozen (preferred) ​60 days
Rejection Criteria
Plasma
​Room Temperature Samples
Interference
​Helolysis and non-fasting status may interfere with results.
Test Information
​Reference intervals and interpretative comments from Gastrointestinal Laboratroy - College of Veterinary Medicine Texas A&M University, College Station, TX 77843-4474
Reference Intervals
Performing Location Reference Intervals
​Marshfield ​Cobalamine: 251-908 ng/L
​Folate: 7.7-24.4- ug/L
​TLI: 5.7-45.2 ug/L
Interpretation

cTLI: <5.7 ug/L - Interpretation: Diagnostic for exocrine pancreatic insufficency (EPI)

cTLI: >45.2 ug/L - Interpretation: Increased serum cTLI.  This may be associated with pancreatitis but has also been reported in association with renal falure or emaciation.

Cobalamin: Canine
251-908 – Interpretation: Result within the reference interval
<251, “<150” – Interpretation: Consistent with distal small intestinal disease, EPI or small intestinal bacterial overgrowth.  Check canine TLI to rule out EPI.  Consider oral or parenteral cobalamin supplementation
251-470 – Interpretation: Result is at the low end of the reference interval.  Suggestive of distal small intestinal disease (i.e., ileum), EPI, or dysbiosis.  Check serum canine TLI concentration to rule out EPI. Oral or parenteral supplementation is indicated.
>908, “>1000” – Interpretation: There is no known clinical significance for serum cobalamin concentrations above the upper limit of the reference interval.
 
Folate: Canine
7.7-24.4 – Interpretation: Result is within the reference interval
<7.7, “<1.0” – Interpretation: Decreased serum folate concentration.  Consistent with disease affecting proximal small intestine.  Consider  folate supplementation.
18.8-24.4 – Interpretation: Upper end of the reference interval.  May reflect small intestinal dysbiosis.  In patients with GI signs but without systemic complications (i.e., sever weight loss, hypoalbuminemia, or other) trial therapies with prebiotics (e.g., a diet containing fructooligosaccharieds (FOS)), probiotics, or antibiotics may be considered.  The Gastrointestinal Laboratory at Texas A & M university recommends Tylosin at 25 mg/kg q 12 hrs. PO for 6-8 weeks.
>24.4, “>50.0” – Interpretation: Consistent with small intestinal dysbiosis.  Small intestinal dybiosis often occurs secondary to chronic enteropathy or various other gastrointestinal disorders but can also be primary (idiopathic).
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield ​Monday through Saturday ​2 hours ​Chemiluminescence/Immulite
Laboratory Section
For most current information refer to the Marshfield Laboratory online reference manual.