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# A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
Flow Cytometry, Veterinary
Test Code: VFLOWC
Synonyms/Keywords
​Flow cytometry; lymphoma/leukemia panel​
Species
Canine, Feline
Test Category
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
​Whole Blood ​EDTA (Lavender Top Tube) ​See Collection Processing Instructions  ​2 mL ​1 mL 

​Solid Tissue (Liver, Spleen, Lymph Nodes, Thymus)

 

​RTT (Red Top Tube plain with no additives), Sterile Transfer Tube or other tube with no additives ​See Collection Processing Instructions ​500 uL of Tissue ​400 uL of Tissue

​Cavitary Fluid (Thoracic, Abdominal, Pericardial)

 

​EDTA (Lavender Top Tube) ​See Collection Processing Instructions ​500 uL of Cavitary Fluid ​400 uL of Cavitary Fluid
Collection Processing Instructions
Collection Processing Instructions
 
For optimum viability, submit samples within 48 hours of collection.  Sample viability can be affected by a variety of factors, including specimen age, temperature/refrigeration, in vivo cell necrosis, etc. If you have questions please call for a consultation with a pathologist prior to sample submission
 
  
Collection Processing Instructions for Whole Blood:
 
1.  Please click to download a flow cytometry submission form. This form provides a space to provide pertinent history and physical exam findings. Please provide as much information as possible, as this is often critical in helping a pathologist provide the best diagnosis possible.  Flow Cytometry Submission Form
 
2.  Results of a CBC (preferably done at Marshfield, although results from analysis at another lab or in house are acceptable) performed optimally within 48 hours from collection of the blood sample helps the pathologist provide the most accurate interpretation of your data. Submission of extra blood for a CBC or attaching data from a CBC performed elsewhere is thus highly recommended.
 
Collection Processing Instructions for Non Blood Solid Tissues:
 
1.  Please click to download a flow cytometry submission form. This form provides a space to provide pertinent history and physical exam findings. Please provide as much information as possible, as this is often critical in helping a pathologist provide the best diagnosis possible.  Flow Cytometry Submission Form
 
2. Place 1 ml saline in a RTT (plain, with no additives). A sterile transfer tube or other similar tube is also acceptable, provided no additives are present. Add 0.1 mL of serum from the same patient or another member of the same species.
Aspirate using suction and expel into the RTT.  Dispel contents into the red topped tube. Rinse syringe and needle with saline in the tube.  Repeat aspiration and rinsing 2-4x if possible.
 
3. Send overnight with a cold pack
 
Collection Processing Instructions for Cavitary Fluids:
 
1.  Please click to download a flow cytometry submission form. This form provides a space to provide pertinent history and physical exam findings. Please provide as much information as possible, as this is often critical in helping a pathologist provide the best diagnosis possible.  Flow Cytometry Submission Form
 
2.  Collect 1 Tube containing a minimum of 400 uL in LTT EDTA tubes.
 
3.  Send overnight with a cold pack
Specimen Stability Information
Specimen Type Temperature Time

​EDTA Whole Blood, Cavitary Fluids, Tissue fluid with saline and serum added

​Refrigerate ​3 to 7 days
Rejection Criteria

Fixed Specimens

Cavitary Fluids and Tissues: Insufficient Volume

Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield ​Monday through Friday, days only ​3-5 days ​Flow Cytometry
Marshfield Center Laboratory Section
Test Information

Flow cytometry enables quantitative evaluation of leukocyte size and immunophenotype. This, along with review of cytology or CBC/blood film by a pathologist allow for determination of lymphocyte identity (i.e T vs. B cell) and significance (i.e. evaluation for leukemia/lymphoma).

Current antibodies tested in dogs include: CD3, CD5(pan-T cell), CD4 (helper T cells), CD8 (cytotoxic T cells),  CD21 (B cells), CD14, CD18 (myelomonocytic cells), CD34 (stem cells), and MHC II.

Current antibodies tested in cats include: CD5(pan-T cell), CD4 (helper T cells), CD8 (cytotoxic T cells),  CD21 (B cells).

Samples with cells of interest <5000 cells/uL in concentration may have inconclusive results due to low cellularity.

Please click to download a flow cytometry submission form. This form provides a space to provide pertinent history and physical exam findings. Please provide as much information as possible, as this is often critical in helping a pathologist provide the most complete diagnosis possible.  Flow Cytometry Submission Form

Reference Intervals
See Report
Synonyms/Keywords
​Flow cytometry; lymphoma/leukemia panel​
Species
Canine, Feline
Test Category
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
​Whole Blood ​EDTA (Lavender Top Tube) ​See Collection Processing Instructions  ​2 mL ​1 mL 

​Solid Tissue (Liver, Spleen, Lymph Nodes, Thymus)

 

​RTT (Red Top Tube plain with no additives), Sterile Transfer Tube or other tube with no additives ​See Collection Processing Instructions ​500 uL of Tissue ​400 uL of Tissue

​Cavitary Fluid (Thoracic, Abdominal, Pericardial)

 

​EDTA (Lavender Top Tube) ​See Collection Processing Instructions ​500 uL of Cavitary Fluid ​400 uL of Cavitary Fluid
Collection Processing Instructions
Collection Processing Instructions
 
For optimum viability, submit samples within 48 hours of collection.  Sample viability can be affected by a variety of factors, including specimen age, temperature/refrigeration, in vivo cell necrosis, etc. If you have questions please call for a consultation with a pathologist prior to sample submission
 
  
Collection Processing Instructions for Whole Blood:
 
1.  Please click to download a flow cytometry submission form. This form provides a space to provide pertinent history and physical exam findings. Please provide as much information as possible, as this is often critical in helping a pathologist provide the best diagnosis possible.  Flow Cytometry Submission Form
 
2.  Results of a CBC (preferably done at Marshfield, although results from analysis at another lab or in house are acceptable) performed optimally within 48 hours from collection of the blood sample helps the pathologist provide the most accurate interpretation of your data. Submission of extra blood for a CBC or attaching data from a CBC performed elsewhere is thus highly recommended.
 
Collection Processing Instructions for Non Blood Solid Tissues:
 
1.  Please click to download a flow cytometry submission form. This form provides a space to provide pertinent history and physical exam findings. Please provide as much information as possible, as this is often critical in helping a pathologist provide the best diagnosis possible.  Flow Cytometry Submission Form
 
2. Place 1 ml saline in a RTT (plain, with no additives). A sterile transfer tube or other similar tube is also acceptable, provided no additives are present. Add 0.1 mL of serum from the same patient or another member of the same species.
Aspirate using suction and expel into the RTT.  Dispel contents into the red topped tube. Rinse syringe and needle with saline in the tube.  Repeat aspiration and rinsing 2-4x if possible.
 
3. Send overnight with a cold pack
 
Collection Processing Instructions for Cavitary Fluids:
 
1.  Please click to download a flow cytometry submission form. This form provides a space to provide pertinent history and physical exam findings. Please provide as much information as possible, as this is often critical in helping a pathologist provide the best diagnosis possible.  Flow Cytometry Submission Form
 
2.  Collect 1 Tube containing a minimum of 400 uL in LTT EDTA tubes.
 
3.  Send overnight with a cold pack
Specimen Stability Information
Specimen Type Temperature Time

​EDTA Whole Blood, Cavitary Fluids, Tissue fluid with saline and serum added

​Refrigerate ​3 to 7 days
Rejection Criteria

Fixed Specimens

Cavitary Fluids and Tissues: Insufficient Volume

Test Information

Flow cytometry enables quantitative evaluation of leukocyte size and immunophenotype. This, along with review of cytology or CBC/blood film by a pathologist allow for determination of lymphocyte identity (i.e T vs. B cell) and significance (i.e. evaluation for leukemia/lymphoma).

Current antibodies tested in dogs include: CD3, CD5(pan-T cell), CD4 (helper T cells), CD8 (cytotoxic T cells),  CD21 (B cells), CD14, CD18 (myelomonocytic cells), CD34 (stem cells), and MHC II.

Current antibodies tested in cats include: CD5(pan-T cell), CD4 (helper T cells), CD8 (cytotoxic T cells),  CD21 (B cells).

Samples with cells of interest <5000 cells/uL in concentration may have inconclusive results due to low cellularity.

Please click to download a flow cytometry submission form. This form provides a space to provide pertinent history and physical exam findings. Please provide as much information as possible, as this is often critical in helping a pathologist provide the most complete diagnosis possible.  Flow Cytometry Submission Form

Reference Intervals
See Report
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield ​Monday through Friday, days only ​3-5 days ​Flow Cytometry
Laboratory Section
For most current information refer to the Marshfield Laboratory online reference manual.