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# A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
Fecal Flotation by Centrifugation & Giardia Antigen
Test Code: VFFG
Synonyms/Keywords
​Ova & Parasites, Antigen, Giardia
Test Components
​VFF and VOPRG
Species
Alpaca, Avian, Bovine, Camelid, Canine, Caprine, Equine, Feline, Ferret, Mouse, Other, Ovine, Porcine, Primate, Rabbit, Rat
Test Category
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
​Fecal Float: Fresh Feces ​50 mL clean container ​3-5 gm (1 Cubic cm or cube approximately 1/2" per side) ​2 gm
​Giardia Antigen: Feces C&S Culture Medium (Cary-Blair)

​Para Pak 10% Formalin

Fresh Stool in a clean, Leak-Proof Container
​One Vial Feces in C&S Culture Medium (Cary-Blair)
Fresh Feces - 5 grams
​​5 grams Stool in C&S Medium or 1 gram Fresh Feces
Collection Processing Instructions

​Fecal Float: Specimens must be fresh and in no preservative.

Giardia Antigen: A portion of fresh stool must be placed in C&S Culture Medium (Cary-Blair) or Para-Pak 10% formalin vial within 24 hours. Refrigerated fresh stool is acceptable.

Specimen Stability Information
Specimen Type Temperature Time
​Fecal Float: Feces ​ ​Refrigerate ​> 6 hours
​Room Temperature ​0-5 hours
​Giardia Antigen: Feces in Cary Blair​ ​​Refrigerate ​​<=7 days
​Giardia Antigen: Fresh Feces ​Refrigerate ​< 48 hours
​Giardia Antigen: Preserved Feces ​Room Temperature ​2 months
Rejection Criteria
Fecal Float Specimens submitted in formalin
​Giardia Antigen ​ ​ ​Fresh feces > 48 hours post-collection
​Feces in Para Pak ZN-PVA fixative
​Leaking specimens
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Cleveland​ ​ ​Fecal Float: Monday through Saturday, excluding holidays ​Less than 2 hours ​Centrifugation & Microscopy
​Giardia Antigen: Monday through Saturday, excluding holidays ​Less than 2 hours ​EIA
​Marshfield ​ ​Fecal Float: Monday through Saturday, excluding holidays ​Less than 2 hours ​Centrifugation & Microscopy
​Giardia Antigen: Monday through Saturday, excluding holidays ​Less than 2 hours ​EIA
​Milwaukee ​Fecal Float: Monday through Saturday, excluding holidays ​Less than 2 hours ​Centrifugation & Microscopy
​Minneapolis ​Fecal Float: Monday through Saturday, excluding holidays ​Less than 2 hours ​Centrifugation & Microscopy
Marshfield Center Laboratory Section
Test Information

​Fecal Float: Observation of coccidia is routinely part of the Fecal Float test.  Low numbers of Cryptosporidium may not be detected with this technique.

Fecal Float: Cryptosporidium, if suspected, should be ordered separately (see Cryptosporidium Antigen)

Giardia Antigen: Formed stool specimens should be thoroughly emulsified and submitted in C&S Medium.  Detects Giardia antigen only.

Reference Intervals
Performing Location Reference Intervals
​Fecal Float: Cleveland, Marshfield, Milwaukee, Minneapolis ​Normal result is Negative
​Giardia Antigen: Cleveland, Marshfield ​Normal result is Negative for Giardia
Interpretation
​​Fecal flotation is used to detect the majority of helminth ova which occur in animal feces.  Most nematode and protozoal ova will be detected by the flotation method.  Fecal sedimentation permits recovery of most trematode (fluke) ova and embryonated ova which are too dense to float.  The Baermann sedimentation method is used to detect larvae in feces.  
Flotation method: Zinc sulfate (specific gravity: 1.181)
Synonyms/Keywords
​Ova & Parasites, Antigen, Giardia
Test Components
​VFF and VOPRG
Species
Alpaca, Avian, Bovine, Camelid, Canine, Caprine, Equine, Feline, Ferret, Mouse, Other, Ovine, Porcine, Primate, Rabbit, Rat
Test Category
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
​Fecal Float: Fresh Feces ​50 mL clean container ​3-5 gm (1 Cubic cm or cube approximately 1/2" per side) ​2 gm
​Giardia Antigen: Feces C&S Culture Medium (Cary-Blair)

​Para Pak 10% Formalin

Fresh Stool in a clean, Leak-Proof Container
​One Vial Feces in C&S Culture Medium (Cary-Blair)
Fresh Feces - 5 grams
​​5 grams Stool in C&S Medium or 1 gram Fresh Feces
Collection Processing Instructions

​Fecal Float: Specimens must be fresh and in no preservative.

Giardia Antigen: A portion of fresh stool must be placed in C&S Culture Medium (Cary-Blair) or Para-Pak 10% formalin vial within 24 hours. Refrigerated fresh stool is acceptable.

Specimen Stability Information
Specimen Type Temperature Time
​Fecal Float: Feces ​ ​Refrigerate ​> 6 hours
​Room Temperature ​0-5 hours
​Giardia Antigen: Feces in Cary Blair​ ​​Refrigerate ​​<=7 days
​Giardia Antigen: Fresh Feces ​Refrigerate ​< 48 hours
​Giardia Antigen: Preserved Feces ​Room Temperature ​2 months
Rejection Criteria
Fecal Float Specimens submitted in formalin
​Giardia Antigen ​ ​ ​Fresh feces > 48 hours post-collection
​Feces in Para Pak ZN-PVA fixative
​Leaking specimens
Test Information

​Fecal Float: Observation of coccidia is routinely part of the Fecal Float test.  Low numbers of Cryptosporidium may not be detected with this technique.

Fecal Float: Cryptosporidium, if suspected, should be ordered separately (see Cryptosporidium Antigen)

Giardia Antigen: Formed stool specimens should be thoroughly emulsified and submitted in C&S Medium.  Detects Giardia antigen only.

Reference Intervals
Performing Location Reference Intervals
​Fecal Float: Cleveland, Marshfield, Milwaukee, Minneapolis ​Normal result is Negative
​Giardia Antigen: Cleveland, Marshfield ​Normal result is Negative for Giardia
Interpretation
​​Fecal flotation is used to detect the majority of helminth ova which occur in animal feces.  Most nematode and protozoal ova will be detected by the flotation method.  Fecal sedimentation permits recovery of most trematode (fluke) ova and embryonated ova which are too dense to float.  The Baermann sedimentation method is used to detect larvae in feces.  
Flotation method: Zinc sulfate (specific gravity: 1.181)
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Cleveland​ ​ ​Fecal Float: Monday through Saturday, excluding holidays ​Less than 2 hours ​Centrifugation & Microscopy
​Giardia Antigen: Monday through Saturday, excluding holidays ​Less than 2 hours ​EIA
​Marshfield ​ ​Fecal Float: Monday through Saturday, excluding holidays ​Less than 2 hours ​Centrifugation & Microscopy
​Giardia Antigen: Monday through Saturday, excluding holidays ​Less than 2 hours ​EIA
​Milwaukee ​Fecal Float: Monday through Saturday, excluding holidays ​Less than 2 hours ​Centrifugation & Microscopy
​Minneapolis ​Fecal Float: Monday through Saturday, excluding holidays ​Less than 2 hours ​Centrifugation & Microscopy
Laboratory Section
For most current information refer to the Marshfield Laboratory online reference manual.