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25447 Culture, Mycobacteria, Other

Culture, Mycobacteria, Other
Test Code: VAF
Synonyms/Keywords
​TB, AFB, acid fast, culture
Test Components
​This test includes a culture and smear exam.
Species
Avian, Bovine, Camelid, Canine, Caprine, Equine, Feline, Ferret, Mouse, Other, Ovine, Porcine, Primate, Rabbit, Rat
Test Category
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
​Any (except blood and bone marrow) ​Sterile leakproof containers without preservatives (e.g. Sage container ​ESwab™, or other Amies medium based swab ​5-10 mL, 1 ESwab, or 3 Amies medium based swabs
Collection Processing Instructions

Specimens should be delivered to the microbiology laboratory as soon as possible. Do not freeze specimens at -20C, or in a frost-free freezer. Specimens should be collected in sterile leakproof containers without preservatives. A sterile anticoagulant such as heparin or SPS may be used in collection tubes to prevent clots in body fluids. Swab-collected specimens and fecal specimens are NOT recommended.

Swabs:

  • ESwab: collect one swab for Gram stain, aerobic and anaerobic cultures, and an additional swab for each additional culture type or stain (e.g. fungus or mycobacteria).
  • Other Amies medium swab types: collect one swab for every culture and stain to be ordered.

See eSwab collection video for more information

For sputum, three samples are recommended (one early morning from deep cough or expectorated sputa induced by aerosolization) eight to twelve hours apart.
For urine, three specimens, one per day for three consecutive days are recommended.
For gastric lavage fluid, three early morning specimens collected on separate days are recommended.
Volumes >15 mL will NOT be accepted.
For blood and bone marrow AFB culture, see Culture, Acid Fast Bacteria, Blood. ​​

Acceptable Body Sites
Bone, Bronchial, Catheter Tip, Environmental, Feces, Fine Needle Aspirate, Fluid, Hardware, Milk, Respiratory Secretions, Swab, Synovial, Tissue, or Urine.
Specimen Stability Information
Specimen Type Temperature Time
​CSF ​Room Temperature ​NA
​All other specimen types ​Refrigerate ​<= 4 days
Rejection Criteria
Pooled sputum or urine​
​Dried swabs or tissues
​Specimens submitted in formalin or other preservatives
​Specimens > 15 mL
​Gastric lavage specimens > 4 hours old or > 15 mL in volume
​Specimens older than 4 days
​Leaking specimens
​​Specimens submitted in Lavender Top Tube (LTT)
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ ​Monday through Friday ​Conventional culture, microscopic exam with mycobacteria-specific stain, standard susceptibility testing, manual or automated identification methods, and nucleic acid sequence analysis as needed.
Marshfield Center Laboratory Section
Test Information

The mycobacterial load in a clinical specimen may be low. Specimens with less than the recommended volumes may therefore lead to a false negative result. Prior antimycobacterial therapy or an inadequate volume of specimen may impact correlation of positive smears with positive cultures. Isolates will receive susceptibility testing only upon provider's request.

‘ESwab’ is a trademark of Copan Diagnostics.
Reference Intervals
Performing Location Reference Intervals
​Marshfield

​Cocentrated/unconcentrated specimen negative for acid-fast bacilli

Culture negative for acid-fast bacilli

Synonyms/Keywords
​TB, AFB, acid fast, culture
Test Components
​This test includes a culture and smear exam.
Species
Avian, Bovine, Camelid, Canine, Caprine, Equine, Feline, Ferret, Mouse, Other, Ovine, Porcine, Primate, Rabbit, Rat
Test Category
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
​Any (except blood and bone marrow) ​Sterile leakproof containers without preservatives (e.g. Sage container ​ESwab™, or other Amies medium based swab ​5-10 mL, 1 ESwab, or 3 Amies medium based swabs
Collection Processing Instructions

Specimens should be delivered to the microbiology laboratory as soon as possible. Do not freeze specimens at -20C, or in a frost-free freezer. Specimens should be collected in sterile leakproof containers without preservatives. A sterile anticoagulant such as heparin or SPS may be used in collection tubes to prevent clots in body fluids. Swab-collected specimens and fecal specimens are NOT recommended.

Swabs:

  • ESwab: collect one swab for Gram stain, aerobic and anaerobic cultures, and an additional swab for each additional culture type or stain (e.g. fungus or mycobacteria).
  • Other Amies medium swab types: collect one swab for every culture and stain to be ordered.

See eSwab collection video for more information

For sputum, three samples are recommended (one early morning from deep cough or expectorated sputa induced by aerosolization) eight to twelve hours apart.
For urine, three specimens, one per day for three consecutive days are recommended.
For gastric lavage fluid, three early morning specimens collected on separate days are recommended.
Volumes >15 mL will NOT be accepted.
For blood and bone marrow AFB culture, see Culture, Acid Fast Bacteria, Blood. ​​

Acceptable Body Sites
Bone, Bronchial, Catheter Tip, Environmental, Feces, Fine Needle Aspirate, Fluid, Hardware, Milk, Respiratory Secretions, Swab, Synovial, Tissue, or Urine.
Specimen Stability Information
Specimen Type Temperature Time
​CSF ​Room Temperature ​NA
​All other specimen types ​Refrigerate ​<= 4 days
Rejection Criteria
Pooled sputum or urine​
​Dried swabs or tissues
​Specimens submitted in formalin or other preservatives
​Specimens > 15 mL
​Gastric lavage specimens > 4 hours old or > 15 mL in volume
​Specimens older than 4 days
​Leaking specimens
​​Specimens submitted in Lavender Top Tube (LTT)
Test Information

The mycobacterial load in a clinical specimen may be low. Specimens with less than the recommended volumes may therefore lead to a false negative result. Prior antimycobacterial therapy or an inadequate volume of specimen may impact correlation of positive smears with positive cultures. Isolates will receive susceptibility testing only upon provider's request.

‘ESwab’ is a trademark of Copan Diagnostics.
Reference Intervals
Performing Location Reference Intervals
​Marshfield

​Cocentrated/unconcentrated specimen negative for acid-fast bacilli

Culture negative for acid-fast bacilli

Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ ​Monday through Friday ​Conventional culture, microscopic exam with mycobacteria-specific stain, standard susceptibility testing, manual or automated identification methods, and nucleic acid sequence analysis as needed.
Laboratory Section
For most current information refer to the Marshfield Laboratory online reference manual.