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25190 Catecholamine Fractionation, Urine-Canine

Catecholamine Fractionation, Urine-Canine
Test Code: VCATRND
Synonyms/Keywords
Norepinephrine, Epinephrine, Dopamine, Adrenalin, Random Norepinephrine/Creatinine Ratio, Epinephrine/Creatinine Ratio, Dopamine/Creatinine Ratio
Test Components

Norepinephrine/Creatinine Ratio

Epinephrine/Creatinine Ratio

Dopamine/Creatinine Ratio

Species
Canine
Test Category
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Urine Random​ Pre-weighed 0.01 g sulfamic acid aliquot tube Any sterile plastic tube or sage cup ​5.0 mL ​3.0 mL
Collection Processing Instructions
Collect urine in a clean container and pour into pre-weighed 0.01 gram sulfamic acid aliquot tube provided by Marshfield Lab. Acceptable alternative preservative is to add a few granules of sulfamic acid or acidify with dilute hydrochloric acid to a pH <5. Acid preservative needs to be added within 24 hours of collection. If preservative is not available, samples can be sent unpreserved and refrigerated if arrival in lab is less than 24 hours from collection time. If time from collection to lab arrival is greater than 24 hours, it is acceptable to immediately freeze unpreserved samples and transport frozen. Unpreserved samples will be acidified upon arrival in lab.
 
A patient control sample does not need to be submitted for this test.
Specimen Stability Information
Specimen Type Temperature Time
​Urine, Random - Preserved ​Refrigerate ​1 month
​Frozen ​2 months
Urine, Random- Not Preserved ​ ​ ​ Refrigerate​ ​24 hours
​Frozen ​2 weeks
Rejection Criteria
Specimens unpreserved and not frozen > 24 hrs.
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield ​Monday and Thursday ​6-8 hours ​UPLC-MS MS (Waters TQ-S system)
Creatinine performed in the 24HS Chemistry Lab; Norepinephrine, Epinephrine, and Dopamine performed in Special Chemistry.
Samples must be in the performing department by 8 am on the day of testing for results the next day.​ ​ ​ ​
Marshfield Center Laboratory Section
Test Information
This test was developed and its performance characteristics determined by Marshfield Labs. This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) to perform high complexity testing.
Reference Intervals
Performing Location Reference Intervals
​Marshfield
Reference Ranges, Canines
 - Norephinephrine/Creatinine ratio = 0.001-0.037 ug/mg Creatinine
 - Epinephrine/Creatinine ratio = 0.000-0.019 ug/mg Creatinine
 - Dopamine/Creatinine ratio = 0.00-0.031 ug/mg Creatinine
Interpretation
Urinary metanephrine/normetanephrine to creatinine ratio (VMETRND) is recommended as the
primary assay to screen for pheochromocytoma.  Catecholamine fractionation (ie catecholamine to creatinine ratio) is also used to diagnose pheochromocytoma. 
 
Urinary concentrations of catecholamines greater than the reference interval support a diagnosis of pheochromocytoma. 
 
 
 
Normetanephrine/creatinine and metanephrine/creatinine results less than 4x the upper
limit of the reference interval DO NOT support a diagnosis of pheochromocytoma.
Normetanephrine/creatinine and/or metanephrine/creatinine results greater than or equal to 4x
the upper limit of the reference interval DO support a diagnosis of pheochromocytoma.
If results are less than 4x the upper limit of the reference interval for the measured
metabolites and a pheochromocytoma remains a differential diagnosis, then consider urinary
epinephrine/norepinephrine/dopamine to creatinine (VCATRND) testing. Please keep in mind
that pheochromocytomas may secrete hormones episodically and therefore even additional
testing may not be confirmatory in some cases of pheochromocytoma.
Synonyms/Keywords
Norepinephrine, Epinephrine, Dopamine, Adrenalin, Random Norepinephrine/Creatinine Ratio, Epinephrine/Creatinine Ratio, Dopamine/Creatinine Ratio
Test Components

Norepinephrine/Creatinine Ratio

Epinephrine/Creatinine Ratio

Dopamine/Creatinine Ratio

Species
Canine
Test Category
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Urine Random​ Pre-weighed 0.01 g sulfamic acid aliquot tube Any sterile plastic tube or sage cup ​5.0 mL ​3.0 mL
Collection Processing Instructions
Collect urine in a clean container and pour into pre-weighed 0.01 gram sulfamic acid aliquot tube provided by Marshfield Lab. Acceptable alternative preservative is to add a few granules of sulfamic acid or acidify with dilute hydrochloric acid to a pH <5. Acid preservative needs to be added within 24 hours of collection. If preservative is not available, samples can be sent unpreserved and refrigerated if arrival in lab is less than 24 hours from collection time. If time from collection to lab arrival is greater than 24 hours, it is acceptable to immediately freeze unpreserved samples and transport frozen. Unpreserved samples will be acidified upon arrival in lab.
 
A patient control sample does not need to be submitted for this test.
Specimen Stability Information
Specimen Type Temperature Time
​Urine, Random - Preserved ​Refrigerate ​1 month
​Frozen ​2 months
Urine, Random- Not Preserved ​ ​ ​ Refrigerate​ ​24 hours
​Frozen ​2 weeks
Rejection Criteria
Specimens unpreserved and not frozen > 24 hrs.
Test Information
This test was developed and its performance characteristics determined by Marshfield Labs. This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) to perform high complexity testing.
Reference Intervals
Performing Location Reference Intervals
​Marshfield
Reference Ranges, Canines
 - Norephinephrine/Creatinine ratio = 0.001-0.037 ug/mg Creatinine
 - Epinephrine/Creatinine ratio = 0.000-0.019 ug/mg Creatinine
 - Dopamine/Creatinine ratio = 0.00-0.031 ug/mg Creatinine
Interpretation
Urinary metanephrine/normetanephrine to creatinine ratio (VMETRND) is recommended as the
primary assay to screen for pheochromocytoma.  Catecholamine fractionation (ie catecholamine to creatinine ratio) is also used to diagnose pheochromocytoma. 
 
Urinary concentrations of catecholamines greater than the reference interval support a diagnosis of pheochromocytoma. 
 
 
 
Normetanephrine/creatinine and metanephrine/creatinine results less than 4x the upper
limit of the reference interval DO NOT support a diagnosis of pheochromocytoma.
Normetanephrine/creatinine and/or metanephrine/creatinine results greater than or equal to 4x
the upper limit of the reference interval DO support a diagnosis of pheochromocytoma.
If results are less than 4x the upper limit of the reference interval for the measured
metabolites and a pheochromocytoma remains a differential diagnosis, then consider urinary
epinephrine/norepinephrine/dopamine to creatinine (VCATRND) testing. Please keep in mind
that pheochromocytomas may secrete hormones episodically and therefore even additional
testing may not be confirmatory in some cases of pheochromocytoma.
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield ​Monday and Thursday ​6-8 hours ​UPLC-MS MS (Waters TQ-S system)
Creatinine performed in the 24HS Chemistry Lab; Norepinephrine, Epinephrine, and Dopamine performed in Special Chemistry.
Samples must be in the performing department by 8 am on the day of testing for results the next day.​ ​ ​ ​
Laboratory Section
For most current information refer to the Marshfield Laboratory online reference manual.