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# A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
Trypsin-Like Immunoreactivity & Pancreatic Lipase Immunreactivity, Canine or Feline
Test Code: VTPLISO
Synonyms/Keywords
​TLI, PLI, EPI (Exocrine Pancreatic Insufficiency)
Test Components
​TLI and PLI
Species
Canine, Feline
Test Category
Specimen Requirements
Fasting Required Specimen Type​ Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)

Yes

12-18 hour fast for non-diabetic patients

6 hour fast for diabetic patients

Serum​​ ​Red Top Tube (RTT) ​Serum Separator Tube (SST) ​1 mL ​0.6 mL
Collection Processing Instructions
​Samples are stable at room temperature and refrigerated for each stated stability, but are preferred frozen as soon as possible.
Specimen Stability Information
Specimen Type Temperature Time
Serum​ ​ ​Room Temperature 2 weeks
Refrigerated 1 month
​Frozen (preferred) Indefinitely
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Texas A&M Gastrointestinal Lab ​Monday through Friday ​1-2 business days

​TLI - RIA

PLI- ELISA

Marshfield Center Laboratory Section
Reference Lab
Reference Lab Information
Address Telephone Website Link Marshfield Lab Account #

College of Veterinary Medicine

​University Street

College Station, TX 77843-4474

​Ph 979-862-2861

Fax 979-862-2864

vetmed.tamu.edu/gilab ​68-026314
Test Information

Interpretation In dogs, TLI values below 2.5 µg/L are diagnostic for EPI. Values between 3.5 and 5.7 µg/L are rarely if ever associated with signs of EPI but may reflect subclinical pancreatic disease such as subtotal pancreatic acinar cell destruction secondary to on-going immune-mediated lymphocytic pancreatitis. Progression of the disease in such patients may ultimately lead to EPI. Values between 2.5 and 3.5 µg/L are sometimes (but rarely) associated with clinical signs due to EPI. In these cases the TLI assay should be repeated after one month paying particular attention to ensuring that food is withheld for 12 to 15 hours before the blood sample is collected.

In cats, TLI values equal to or below 8.0 µg/L are diagnostic for EPI, with values between 8.0 and 12.0 being equivocal. As in the dog, repeating the assay one month later should be considered.

Serum TLI values above 50.0 µg/L (dogs) and 100.0 µg/L (cats) are consistent with either acute or chronic pancreatitis or decreased renal excretion due to severe renal insufficiency, although our experience suggests that serum TLI is often minimally increased even in severe renal failure. Elevated serum TLI concentrations are also seen in some malnourished patients (dogs usually) without evidence of pancreatitis, and in some cats with patchy pancreatic hypertrophy/atrophy (generally considered to be a benign age-related change). Serum TLI is increased in approximately 30-40% of cats and dogs with pancreatitis; it is important to recognize that normal test results do not rule out the possibility of pancreatic inflammation. We believe that in acute pancreatitis testing of samples obtained as soon as possible after the onset of clinical signs is most likely to yield an abnormal test result. If pancreatitis is suspected, a PLI test should be performed. In cats increased serum TLI is often also observed with small intestinal disease. In these cases serum concentrations of cobalamin and folate should be determined for evaluation of the small intestine.

Reference Intervals
Performing Location Canine Reference Interval​ ​Feline Reference Interval
Texas A&M Gastrointestinal Lab​

​TLI:  5.7-45.2 ug/L

PLI:  0-200 ug/L

​TLI:  12.0-82.0 ug/mL

PLI:  0-3.5 ug/L

Synonyms/Keywords
​TLI, PLI, EPI (Exocrine Pancreatic Insufficiency)
Test Components
​TLI and PLI
Species
Canine, Feline
Test Category
Specimen Requirements
Fasting Required Specimen Type​ Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)

Yes

12-18 hour fast for non-diabetic patients

6 hour fast for diabetic patients

Serum​​ ​Red Top Tube (RTT) ​Serum Separator Tube (SST) ​1 mL ​0.6 mL
Collection Processing Instructions
​Samples are stable at room temperature and refrigerated for each stated stability, but are preferred frozen as soon as possible.
Specimen Stability Information
Specimen Type Temperature Time
Serum​ ​ ​Room Temperature 2 weeks
Refrigerated 1 month
​Frozen (preferred) Indefinitely
Test Information

Interpretation In dogs, TLI values below 2.5 µg/L are diagnostic for EPI. Values between 3.5 and 5.7 µg/L are rarely if ever associated with signs of EPI but may reflect subclinical pancreatic disease such as subtotal pancreatic acinar cell destruction secondary to on-going immune-mediated lymphocytic pancreatitis. Progression of the disease in such patients may ultimately lead to EPI. Values between 2.5 and 3.5 µg/L are sometimes (but rarely) associated with clinical signs due to EPI. In these cases the TLI assay should be repeated after one month paying particular attention to ensuring that food is withheld for 12 to 15 hours before the blood sample is collected.

In cats, TLI values equal to or below 8.0 µg/L are diagnostic for EPI, with values between 8.0 and 12.0 being equivocal. As in the dog, repeating the assay one month later should be considered.

Serum TLI values above 50.0 µg/L (dogs) and 100.0 µg/L (cats) are consistent with either acute or chronic pancreatitis or decreased renal excretion due to severe renal insufficiency, although our experience suggests that serum TLI is often minimally increased even in severe renal failure. Elevated serum TLI concentrations are also seen in some malnourished patients (dogs usually) without evidence of pancreatitis, and in some cats with patchy pancreatic hypertrophy/atrophy (generally considered to be a benign age-related change). Serum TLI is increased in approximately 30-40% of cats and dogs with pancreatitis; it is important to recognize that normal test results do not rule out the possibility of pancreatic inflammation. We believe that in acute pancreatitis testing of samples obtained as soon as possible after the onset of clinical signs is most likely to yield an abnormal test result. If pancreatitis is suspected, a PLI test should be performed. In cats increased serum TLI is often also observed with small intestinal disease. In these cases serum concentrations of cobalamin and folate should be determined for evaluation of the small intestine.

Reference Intervals
Performing Location Canine Reference Interval​ ​Feline Reference Interval
Texas A&M Gastrointestinal Lab​

​TLI:  5.7-45.2 ug/L

PLI:  0-200 ug/L

​TLI:  12.0-82.0 ug/mL

PLI:  0-3.5 ug/L

Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Texas A&M Gastrointestinal Lab ​Monday through Friday ​1-2 business days

​TLI - RIA

PLI- ELISA

Laboratory Section
Reference Lab
Reference Lab Information
Address Telephone Website Link Marshfield Lab Account #

College of Veterinary Medicine

​University Street

College Station, TX 77843-4474

​Ph 979-862-2861

Fax 979-862-2864

vetmed.tamu.edu/gilab ​68-026314
For most current information refer to the Marshfield Laboratory online reference manual.