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26500 Orthopoxvirus Molecular Detection PCR, Swab (MPXDX)

Orthopoxvirus Molecular Detection PCR, Swab (MPXDX)
Test Code: MPXDXSO
Synonyms/Keywords

Monkeypox
Monkeypox virus

Orthopoxvirus

Non-variola

Vaccinia

Useful For

Detection of Orthopoxviruses in patients with suspect monkeypox virus infection.

Patients must have lesions and symptoms consistent with monkeypox. 

Refer to the MCHS Monkeypox site including Patient Testing and Infectious Disease Alert links for detailed guidance. For more information on clinical presentation refer to: CDC Monkeypox Clinical Recognition

Notify your local MCHS Infection Preventionist of a suspect case. Confirmed and suspected monkeypox disease is a Category 1 reportable condition in Wisconsin.

Specimen Requirements
Specimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​Dry Swab of Skin LesionSubmit two dry swabs in a single sterile, screw-capped container​2 swabs​2 swabs​2 swabs
Collection Processing Instructions

NECESSARY INFORMATION

Specimen source with anatomical location is required.

 

Notify your Infection Preventionist and Local Health Department of a suspect case being tested.

Submit two dry swab collections from a skin lesion by rubbing vigorously over the lesion and placing both swabs in a single sterile container. Swabs must be polyester, nylon or Dacron with a plastic shaft. Do not submit swabs in universal transport media. Comment the specific body site swabbed on the order.

Deliver specimens to the laboratory as soon as possible on ice.

Upon arrival in lab, notify Mary Stemper or Dr. Novicki in Microbiology Section.  Any additional specimen manipulations must be performed within a biological safety cabinet using enhanced BSL-3 practices.

Specimens can be stored and transported to Mayo at 2-8 degrees C (refrigerated/with ice packs). Specimens may also be frozen and shipped on dry ice.  

Specimen Stability Information
Specimen TypeTemperatureTime
​Swab ​​Refrigerated (preferred)​7 days
​Frozen​60 days
Rejection Criteria

Swab submission tubes containing foam, gel, charcoal, or any liquid besides viral transport media
Glass transport tubes
Swabs submitted in paper swab packaging sleeve
Swabs submitted in UTM (universal transport media)
Wood swabs
Foam tipped swabs​
Cotton swabs

Specimens received ≥8°C

 Bloody specimens

Reject
ThawedCold OK; Warm reject
Performing Laboratory Information
Performing LocationDay(s) Test PerformedReport AvailableMethodology/Instrumentation
​Mayo Clinic Laboratories​Monday through Friday​2 to 5 days​Real-Time Polymerase Chain Reaction (PCR)
Reference Lab
Test Information

​Mayo Laboratories uses an RT-PCR test for non-variola orthopox viruses using CDC LRN protocols.

Specimens that test positive for a non-variola orthopox virus will be reported to public health and the CDC.

Reference Range Information
Performing LocationReference Range
​Mayo Clinic Laboratories​Undetected
Interpretation

A negative test result does not rule out the possibility of infectious.

A positive result is currently presumptive for West African clade Monkeypox, due to the ongoing outbreak.

This RT-PCR assay can detect DNA from orthopox viruses including monkeypox, vaccinia, and cowpox; it does not detect variola virus (smallpox).  The test was developed by the Centers of Disease Control and Prevention and its performance characteristics validated at Mayo.  It has been cleared by the U.S. Food and Drug Administration.

Outreach CPTs
CPTModifier
(if needed)
QuantityDescriptionComments
​87593​1
Synonyms/Keywords

Monkeypox
Monkeypox virus

Orthopoxvirus

Non-variola

Vaccinia

Ordering Applications
Ordering ApplicationDescription
​COM​Orthopoxvirus PCR (MPXDX)
​Cerner​Orthopoxvirus PCR (MPXDX)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​Dry Swab of Skin LesionSubmit two dry swabs in a single sterile, screw-capped container​2 swabs​2 swabs​2 swabs
Collection Processing

NECESSARY INFORMATION

Specimen source with anatomical location is required.

 

Notify your Infection Preventionist and Local Health Department of a suspect case being tested.

Submit two dry swab collections from a skin lesion by rubbing vigorously over the lesion and placing both swabs in a single sterile container. Swabs must be polyester, nylon or Dacron with a plastic shaft. Do not submit swabs in universal transport media. Comment the specific body site swabbed on the order.

Deliver specimens to the laboratory as soon as possible on ice.

Upon arrival in lab, notify Mary Stemper or Dr. Novicki in Microbiology Section.  Any additional specimen manipulations must be performed within a biological safety cabinet using enhanced BSL-3 practices.

Specimens can be stored and transported to Mayo at 2-8 degrees C (refrigerated/with ice packs). Specimens may also be frozen and shipped on dry ice.  

Specimen Stability Information
Specimen TypeTemperatureTime
​Swab ​​Refrigerated (preferred)​7 days
​Frozen​60 days
Rejection Criteria

Swab submission tubes containing foam, gel, charcoal, or any liquid besides viral transport media
Glass transport tubes
Swabs submitted in paper swab packaging sleeve
Swabs submitted in UTM (universal transport media)
Wood swabs
Foam tipped swabs​
Cotton swabs

Specimens received ≥8°C

 Bloody specimens

Reject
ThawedCold OK; Warm reject
Useful For

Detection of Orthopoxviruses in patients with suspect monkeypox virus infection.

Patients must have lesions and symptoms consistent with monkeypox. 

Refer to the MCHS Monkeypox site including Patient Testing and Infectious Disease Alert links for detailed guidance. For more information on clinical presentation refer to: CDC Monkeypox Clinical Recognition

Notify your local MCHS Infection Preventionist of a suspect case. Confirmed and suspected monkeypox disease is a Category 1 reportable condition in Wisconsin.

Reference Range Information
Performing LocationReference Range
​Mayo Clinic Laboratories​Undetected
Interpretation

A negative test result does not rule out the possibility of infectious.

A positive result is currently presumptive for West African clade Monkeypox, due to the ongoing outbreak.

This RT-PCR assay can detect DNA from orthopox viruses including monkeypox, vaccinia, and cowpox; it does not detect variola virus (smallpox).  The test was developed by the Centers of Disease Control and Prevention and its performance characteristics validated at Mayo.  It has been cleared by the U.S. Food and Drug Administration.

For more information visit:
Performing Laboratory Information
Performing LocationDay(s) Test PerformedReport AvailableMethodology/Instrumentation
​Mayo Clinic Laboratories​Monday through Friday​2 to 5 days​Real-Time Polymerase Chain Reaction (PCR)
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPTModifier
(if needed)
QuantityDescriptionComments
​87593​1
For most current information refer to the Marshfield Laboratory online reference manual.