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26491 Human Papillomavirus (HPV), High-Risk, DNA In Situ Hybridization (HPVHR)

Human Papillomavirus (HPV), High-Risk, DNA In Situ Hybridization (HPVHR)
Test Code: HPVHRSO
Synonyms/Keywords

​HPV (Human Papillomavirus), HPVHR, GPHPVHRISH, ISHPVFAM16ISH, ISNEGHPVISH, ISDNAHPVISH

Test Components

The probe set used in this human papillomavirus (HPV) DNA in situ hybridization (ISH) test cannot detect all potential HPV serotypes that are associated with oropharyngeal squamous cell carcinoma. If this test is negative, a more sensitive test, Human Papillomavirus (HPV) High Risk E6/E7 RNA In Situ Hybridization, could be performed and reported if clinically indicated but will not be billed separately.​

Useful For

​Detection of human papillomavirus DNA from high-risk genotypes (16, 18, 31, 33, and 51)

Specimen Requirements
Specimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)		Pediatric Minimum Volume
(no repeat)
​Tissue​Formalin-fixed, paraffin-embedded tissue block​​Entire Block​​
​Slides​5 unstained glass, positively charged slides with 5 (+ or - 1)-microns formalin-fixed, paraffin-embedded tissue​​5 slides​​
Collection Processing Instructions

​A pathology/diagnostic report and brief history are required. 

Specimen Stability Information
Specimen TypeTemperature
​Special ​​Ambient (preferred)
​Refrigerated
Rejection Criteria
Wet/frozen tissue
​Cytology smears
​Nonformalin fixed tissue
​Nonparaffin embedded tissue
​Noncharged slides
​ProbeOn slides
Interference

​Age of a cut paraffin section can affect staining quality. Stability thresholds vary widely among published literature. Best practice is for paraffin sections to be cut within 6 weeks.

Performing Laboratory Information
Performing LocationDay(s) Test PerformedReport AvailableMethodology/Instrumentation
​Mayo Clinic Laboratories​Monday through Friday​5 to 7 days​In Situ Hybridization (ISH)
Reference Lab
Mayo Clinic Laboratories
Test Information

​Persistent infections with high-risk human papillomavirus (HPV) genotypes (16, 18, 31, 33, and 51) are associated with cervical, vaginal, vulvar, and head and neck malignancies. Patients with HPV-related oropharyngeal squamous cell carcinoma (OPSCC) have shown better disease-specific survival and overall survival when compared to HPV-negative cases of OPSCC.

Interpretation

​This test, when not accompanied by a pathology consultation request, will be answered as either positive or negative. If additional interpretation or analysis is needed, request PATHC / Pathology Consultation along with this test.

Outreach CPTs
CPTModifier
(if needed)		QuantityDescriptionComments
​88365​​1​Primary​
​88364​​1 ​If additional ISH​if needed
Synonyms/Keywords

​HPV (Human Papillomavirus), HPVHR, GPHPVHRISH, ISHPVFAM16ISH, ISNEGHPVISH, ISDNAHPVISH

Test Components

The probe set used in this human papillomavirus (HPV) DNA in situ hybridization (ISH) test cannot detect all potential HPV serotypes that are associated with oropharyngeal squamous cell carcinoma. If this test is negative, a more sensitive test, Human Papillomavirus (HPV) High Risk E6/E7 RNA In Situ Hybridization, could be performed and reported if clinically indicated but will not be billed separately.​

Ordering Applications
Ordering ApplicationDescription
​COM​Surgical Pathology, HPV High Risk DNA ISH (HPVHR)
​Cerner​HPV High-Risk, DNA ISH (HPVHR)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)		Pediatric Minimum Volume
(no repeat)
​Tissue​Formalin-fixed, paraffin-embedded tissue block​​Entire Block​​
​Slides​5 unstained glass, positively charged slides with 5 (+ or - 1)-microns formalin-fixed, paraffin-embedded tissue​​5 slides​​
Collection Processing

​A pathology/diagnostic report and brief history are required. 

Specimen Stability Information
Specimen TypeTemperature
​Special ​​Ambient (preferred)
​Refrigerated
Rejection Criteria
Wet/frozen tissue
​Cytology smears
​Nonformalin fixed tissue
​Nonparaffin embedded tissue
​Noncharged slides
​ProbeOn slides
Interference

​Age of a cut paraffin section can affect staining quality. Stability thresholds vary widely among published literature. Best practice is for paraffin sections to be cut within 6 weeks.

Useful For

​Detection of human papillomavirus DNA from high-risk genotypes (16, 18, 31, 33, and 51)

Test Components

The probe set used in this human papillomavirus (HPV) DNA in situ hybridization (ISH) test cannot detect all potential HPV serotypes that are associated with oropharyngeal squamous cell carcinoma. If this test is negative, a more sensitive test, Human Papillomavirus (HPV) High Risk E6/E7 RNA In Situ Hybridization, could be performed and reported if clinically indicated but will not be billed separately.​

Interpretation

​This test, when not accompanied by a pathology consultation request, will be answered as either positive or negative. If additional interpretation or analysis is needed, request PATHC / Pathology Consultation along with this test.

For more information visit:
Performing Laboratory Information
Performing LocationDay(s) Test PerformedReport AvailableMethodology/Instrumentation
​Mayo Clinic Laboratories​Monday through Friday​5 to 7 days​In Situ Hybridization (ISH)
Reference Lab
Mayo Clinic Laboratories
For billing questions, see Contacts
Outreach CPTs
CPTModifier
(if needed)		QuantityDescriptionComments
​88365​​1​Primary​
​88364​​1 ​If additional ISH​if needed
For most current information refer to the Marshfield Laboratory online reference manual.