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26445 HLA-B27 Determination (2271)

HLA-B27 Determination (2271)
Test Code: HLA27SO
Synonyms/Keywords

​HLA-27, PCR (2271), HLA-B27 Determination

Useful For

​Test for disorders associated with HLA-B27 allele such as Spondyloarthritis.

Specimen Requirements
Fasting RequiredSpecimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)		Pediatric Minimum Volume
(no repeat)
​​Buccal Swab​Buccal Swab​​4 Buccal Swabs​3-4 Buccal Swabs​3-4 Buccal Swabs
​​EDTA Whole Blood​Lavender Top Tube EDTA​​14 mL​5 mL​5 mL
Collection Processing Instructions

​Indicate on specimen tube and requistion whether sample is whole blood or cord blood. Contact Versiti if sending cord blood or DNA.

Send only 14 mL EDTA whole blood or 4 buccal swabs if more than one HLA typing test is ordered.

Specimen Stability Information
Specimen TypeTemperatureTime
​Buccal SwabAmbient​
​EDTA Whole Blood​Ambient​
Performing Laboratory Information
Performing LocationDay(s) Test PerformedAnalytical TimeMethodology/Instrumentation
​Versiti​​5-7 Days​PCR-rSSO, -SSP (melt curve analysis), and/or Direct DNA Sequencing
Reference Lab
Versiti/Blood Center of Wisconsin
Reference Range Information
Performing LocationReference Range
​Versiti​Interpretive Report
Outreach CPTs
CPTModifier
(if needed)		QuantityDescriptionComments
81374​​​1​​
Synonyms/Keywords

​HLA-27, PCR (2271), HLA-B27 Determination

Ordering Applications
Ordering ApplicationDescription
​Cerner​HLA-27, PCR (2271)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting RequiredSpecimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)		Pediatric Minimum Volume
(no repeat)
​​Buccal Swab​Buccal Swab​​4 Buccal Swabs​3-4 Buccal Swabs​3-4 Buccal Swabs
​​EDTA Whole Blood​Lavender Top Tube EDTA​​14 mL​5 mL​5 mL
Collection Processing

​Indicate on specimen tube and requistion whether sample is whole blood or cord blood. Contact Versiti if sending cord blood or DNA.

Send only 14 mL EDTA whole blood or 4 buccal swabs if more than one HLA typing test is ordered.

Specimen Stability Information
Specimen TypeTemperatureTime
​Buccal SwabAmbient​
​EDTA Whole Blood​Ambient​
Useful For

​Test for disorders associated with HLA-B27 allele such as Spondyloarthritis.

Reference Range Information
Performing LocationReference Range
​Versiti​Interpretive Report
For more information visit:
Performing Laboratory Information
Performing LocationDay(s) Test PerformedAnalytical TimeMethodology/Instrumentation
​Versiti​​5-7 Days​PCR-rSSO, -SSP (melt curve analysis), and/or Direct DNA Sequencing
Reference Lab
Versiti/Blood Center of Wisconsin
For billing questions, see Contacts
Outreach CPTs
CPTModifier
(if needed)		QuantityDescriptionComments
81374​​​1​​
For most current information refer to the Marshfield Laboratory online reference manual.