M-Protein Isotype, Serum (MALD), Immunofixation, Immunosubtraction, Isotyping, Mass-Fix, miRAMM, Multiple Myeloma, MassFix
Aiding in the diagnosis of monoclonal gammopathies, when used in conjuction with free light chain studies
This test alone is not considered an adequate screen for monoclonal gammopathies.
No significant cautionary statements
Immunotyping of monoclonal (M-) proteins identifies the monoclonal immunoglobulin heavy chain type (gamma, alpha, mu, delta, or epsilon) and light chain type (kappa or lambda) in serum specimens.
A characteristic monoclonal band (M-spike) is often found on serum protein electrophoresis (SPE) in the gamma globulin region and, more rarely, in the beta or alpha-2 regions. The finding of an M-spike, restricted migration, or hypogammaglobulinemic protein electrophoresis pattern is suggestive of a possible monoclonal protein.
Immunoaffinity purification followed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) is performed to identify the immunoglobulin heavy and light chains.