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26342 Histoplasma Antibody, Serum (SHSTO)

Histoplasma Antibody, Serum (SHSTO)
Test Code: SHSTOSO
Synonyms/Keywords

​Fungal serology, Histo, Histoplasma capsulatum, Histoplasma Complement Fixation, Histoplasmosis, Immunodiffusion Serology for Fungi

Useful For

​Aiding in the diagnosis of active histoplasmosis using serum specimens

Specimen Requirements
Specimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen Volume
​Serum​Serum Separator Tube (SST)​Red Top Tube (RTT)​0.5 mL
Specimen Stability Information
Specimen TypeTemperatureTime
​Serum ​​Refrigerated (preferred)​14 days
​Frozen​14 days
Rejection Criteria

Gross Hemolysis

Gross Lipemia

Interference

Recent histoplasmosis skin tests must be avoided because the test causes a misleading rise in complement fixation titer, as well as an M precipitin band, in approximately 17% of patients having previous exposure to Histoplasma capsulatum.

Cross-reacting antibodies sometimes present interpretive problems in patients having blastomycosis or coccidioidomycosis.

Performing Laboratory Information
Performing LocationDay(s) Test PerformedAnalytical TimeMethodology/Instrumentation
Mayo Clinic Laboratories​​Monday through Friday​2-7 days​Complement Fixation (CF)/ Immunodiffusion
Reference Lab
Test Information

​Histoplasma capsulatum is a soil saprophyte that grows well in soil enriched with bird droppings. The usual disease is self-limited, affects the lungs, and is asymptomatic. Chronic cavitary pulmonary disease, disseminated disease, and meningitis may occur and can be fatal, especially in young children and in immunosuppressed patients.

Reference Range Information
Performing LocationReference Range
​Mayo Clinic Laboratories

MYCELIAL BY COMPLEMENT FIXATION (CF)

Negative (positives reported as titer)

 

YEAST BY CF

Negative (positives reported as titer)

 

ANTIBODY BY IMMUNODIFFUSION

Negative (positives reported as band present)

Interpretation

​Complement fixation (CF) titers of 1:32 or higher indicate active disease. A rising CF titer is associated with progressive infection.

 

Positive immunodiffusion test results supplement findings of the CF test. The simultaneous appearance of both H and M precipitin bands indicates active histoplasmosis. The M precipitin band alone indicates early or chronic disease or a recent histoplasmosis skin test.

 

Patients infected with Histoplasma capsulatum demonstrate a serum antibody with a rising titer within 6 weeks of infection. A rising titer is associated with progressive infection. Specific antibody persists for a few weeks to a year, regardless of clinical improvement.

Outreach CPTs
CPTModifier
(if needed)
QuantityDescriptionComments
​86698​3
Synonyms/Keywords

​Fungal serology, Histo, Histoplasma capsulatum, Histoplasma Complement Fixation, Histoplasmosis, Immunodiffusion Serology for Fungi

Ordering Applications
Ordering ApplicationDescription
​Cerner​Histoplasma Antibody
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen Volume
​Serum​Serum Separator Tube (SST)​Red Top Tube (RTT)​0.5 mL
Specimen Stability Information
Specimen TypeTemperatureTime
​Serum ​​Refrigerated (preferred)​14 days
​Frozen​14 days
Rejection Criteria

Gross Hemolysis

Gross Lipemia

Interference

Recent histoplasmosis skin tests must be avoided because the test causes a misleading rise in complement fixation titer, as well as an M precipitin band, in approximately 17% of patients having previous exposure to Histoplasma capsulatum.

Cross-reacting antibodies sometimes present interpretive problems in patients having blastomycosis or coccidioidomycosis.

Useful For

​Aiding in the diagnosis of active histoplasmosis using serum specimens

Reference Range Information
Performing LocationReference Range
​Mayo Clinic Laboratories

MYCELIAL BY COMPLEMENT FIXATION (CF)

Negative (positives reported as titer)

 

YEAST BY CF

Negative (positives reported as titer)

 

ANTIBODY BY IMMUNODIFFUSION

Negative (positives reported as band present)

Interpretation

​Complement fixation (CF) titers of 1:32 or higher indicate active disease. A rising CF titer is associated with progressive infection.

 

Positive immunodiffusion test results supplement findings of the CF test. The simultaneous appearance of both H and M precipitin bands indicates active histoplasmosis. The M precipitin band alone indicates early or chronic disease or a recent histoplasmosis skin test.

 

Patients infected with Histoplasma capsulatum demonstrate a serum antibody with a rising titer within 6 weeks of infection. A rising titer is associated with progressive infection. Specific antibody persists for a few weeks to a year, regardless of clinical improvement.

For more information visit:
Performing Laboratory Information
Performing LocationDay(s) Test PerformedAnalytical TimeMethodology/Instrumentation
Mayo Clinic Laboratories​​Monday through Friday​2-7 days​Complement Fixation (CF)/ Immunodiffusion
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPTModifier
(if needed)
QuantityDescriptionComments
​86698​3
For most current information refer to the Marshfield Laboratory online reference manual.