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26205 Tobramycin, Peak, Serum (TOBPA)

Tobramycin, Peak, Serum (TOBPA)
Test Code: TOBPASO
Synonyms/Keywords

​Antibiotic Assay
Antimicrobial Assay, Tobramycin
Nebcin (Tobramycin Sulfate Injection)
Tobramycin, Antimicrobial Assay

Useful For

​Monitoring adequacy of serum concentration during tobramycin therapy

Specimen Requirements
Specimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​Serum​Serum Separator Tube (SST)​Red Top Tube (RTT)​0.5 mL​0.25 mL
Collection Processing Instructions

1. Serum for a peak level should be drawn 30 to 60 minutes after last dose.

2. Serum gel tubes should be centrifuged within 2 hours of collection.

3. Red-top tubes should be centrifuged and aliquoted within 2 hours of collection.

Specimen Stability Information
Specimen TypeTemperatureTime
Serum​ ​ ​​Refrigerated (preferred)​7 days
​Frozen​28 days
​Ambient​72 hours
Rejection Criteria
Gross Hemolysis
Performing Laboratory Information
Performing LocationDay(s) Test PerformedAnalytical TimeMethodology/Instrumentation
​Mayo Clinic Laboratories​Monday through Sunday​1-2 days​Immunoassay
Reference Lab
Test Information

​Tobramycin is an antibiotic used to treat life-threatening blood infections caused by gram-negative bacilli, particularly Citrobacter freundii, Enterobacter (all species), Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Providencia stuartii, Pseudomonas aeruginosa, and Serratia species. It is often used in combination with beta-lactam therapy.

A tobramycin minimum inhibitory concentration (MIC) of less than 4.0 mcg/mL is considered susceptible for gram-negative bacilli, while a MIC of greater than 8.0 mcg/mL is considered resistant.

Toxicities include ototoxicity and nephrotoxicity. This risk is enhanced in presence of other ototoxic or nephrotoxic drugs. Monitoring of serum levels, renal function, and symptoms consistent with ototoxicity is important. For longer durations of use, audiology and vestibular testing should be considered at baseline and periodically during therapy.

Reference Range Information
Performing LocationReference Range
​Mayo Clinic Laboratories

Therapeutic: 3.0-12.0 mcg/mL

Toxic: >12.0 mcg/mL

Interpretation

​Target peak concentrations depend on the type of infection being treated. Peak levels for most infections using conventional dosing are 3.0 to 12.0 mcg/mL. Prolonged exposure to peak concentrations exceeding 12.0 mcg/mL may lead to toxicity.

Outreach CPTs
CPTModifier
(if needed)
QuantityDescriptionComments
​80200​1
Synonyms/Keywords

​Antibiotic Assay
Antimicrobial Assay, Tobramycin
Nebcin (Tobramycin Sulfate Injection)
Tobramycin, Antimicrobial Assay

Ordering Applications
Ordering ApplicationDescription
​COM​Tobramycin, Peak, Serum
​Cerner​Tobramycin, Peak, S (TOBPA)
​Centricity​Tobramycin, Peak, S (TOBPA)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen TypePreferred Container/TubeAcceptable Container/TubeSpecimen VolumeSpecimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​Serum​Serum Separator Tube (SST)​Red Top Tube (RTT)​0.5 mL​0.25 mL
Collection Processing

1. Serum for a peak level should be drawn 30 to 60 minutes after last dose.

2. Serum gel tubes should be centrifuged within 2 hours of collection.

3. Red-top tubes should be centrifuged and aliquoted within 2 hours of collection.

Specimen Stability Information
Specimen TypeTemperatureTime
Serum​ ​ ​​Refrigerated (preferred)​7 days
​Frozen​28 days
​Ambient​72 hours
Rejection Criteria
Gross Hemolysis
Useful For

​Monitoring adequacy of serum concentration during tobramycin therapy

Reference Range Information
Performing LocationReference Range
​Mayo Clinic Laboratories

Therapeutic: 3.0-12.0 mcg/mL

Toxic: >12.0 mcg/mL

Interpretation

​Target peak concentrations depend on the type of infection being treated. Peak levels for most infections using conventional dosing are 3.0 to 12.0 mcg/mL. Prolonged exposure to peak concentrations exceeding 12.0 mcg/mL may lead to toxicity.

For more information visit:
Performing Laboratory Information
Performing LocationDay(s) Test PerformedAnalytical TimeMethodology/Instrumentation
​Mayo Clinic Laboratories​Monday through Sunday​1-2 days​Immunoassay
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPTModifier
(if needed)
QuantityDescriptionComments
​80200​1
For most current information refer to the Marshfield Laboratory online reference manual.