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26120 Mycoplasma genitalium, PCR (MGRP)

Mycoplasma genitalium, PCR (MGRP)
Test Code: MGRPSO
Synonyms/Keywords

Mycoplasma genitalium

Mycoplasmoides genitalium

Useful For
Detection of Mycoplasma genitalium from genitourinary and reproductive sources.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​Urogenital, Vaginal, Cervix, Urethra ​M6 Transport Media ​M4 or M5 Transport Media, Swab in Transport Media, M4-RT, Culturette (BBL Culture Swab), Culture swab transport system (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium), Universal transport medium, Eswab ​One swab ​One swab
​Fluids, Amniotic, Pelvic, Prostatic, Semen ​Sterile, screw-capped container ​M4, M5 or M6 Transport Media ​2 mL ​1 mL
​Urine (Urine first void: Specimen can be collected at any time during the day. The patient should not have urinated for at least 1 hour prior to specimen collection. The first voided portion is the initial 20-30 mL of the urine stream obtained without cleaning the external urethra.) ​Sterile, screw-capped container ​10 mL ​2 mL
​Tissue (Fresh), Placenta, Genitourinary ​​Sterile, screw-capped container ​5 mm(3)
Specimen Stability Information
Specimen Type Temperature Time
Varies ​ ​Refrigerate (preferred) ​7 days
​Frozen ​7 days
Rejection Criteria
Cotton or calcium alginate-tipped swab, wooden shaft swab, dry swabs, swab containing gel or charcoal
​Formalin-fixed and/or paraffin-embedded tissues
​Port-a-Cul tube
​Anaerobic fluid vials
Interference

​Interfering substances may affect the accuracy of this assay; results should always be interpreted in conjunction with clinical and epidemiological findings.

This test does not detect other Mycoplasmoides or ureaplasmas.

Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Clinic Laboratories ​Monday through Friday ​3-4 days Real-Time Polymerase Chain Reaction (PCR) using LightCycler and Fluorescent Resonance Energy Transfer (FRET) 
Reference Lab
Test Information

A positive PCR result for the presence of a specific sequence found within the Mycoplasmoides genitalium tuf gene indicates the presence of M genitalium DNA in the specimen.

A negative PCR result indicates the absence of detectable M genitalium DNA in the specimen, it but does not rule-out infection as false-negative results may occur due to the following; inhibition of PCR, sequence variability underlying the primers or probes, or the presence of M genitalium in quantities below the limit of detection of the assay.

 
Interpretation

A positive PCR result for the presence of a specific sequence found within the Mycoplasmoides genitalium tuf gene indicates the presence of M genitalium DNA in the specimen.

A negative PCR result indicates the absence of detectable M genitalium DNA in the specimen, it but does not rule-out infection as false-negative results may occur due to the following; inhibition of PCR, sequence variability underlying the primers or probes, or the presence of M genitalium in quantities below the limit of detection of the assay.

Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​87563 ​1
Synonyms/Keywords

Mycoplasma genitalium

Mycoplasmoides genitalium

Ordering Applications
Ordering Application Description
​Centricity Mycoplasma genitalium, PCR (MGRP)
​Cerner Mycoplasma genitalium, PCR (MGRP)
​COM Mycoplasma genitalium, PCR (MGRP)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​Urogenital, Vaginal, Cervix, Urethra ​M6 Transport Media ​M4 or M5 Transport Media, Swab in Transport Media, M4-RT, Culturette (BBL Culture Swab), Culture swab transport system (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium), Universal transport medium, Eswab ​One swab ​One swab
​Fluids, Amniotic, Pelvic, Prostatic, Semen ​Sterile, screw-capped container ​M4, M5 or M6 Transport Media ​2 mL ​1 mL
​Urine (Urine first void: Specimen can be collected at any time during the day. The patient should not have urinated for at least 1 hour prior to specimen collection. The first voided portion is the initial 20-30 mL of the urine stream obtained without cleaning the external urethra.) ​Sterile, screw-capped container ​10 mL ​2 mL
​Tissue (Fresh), Placenta, Genitourinary ​​Sterile, screw-capped container ​5 mm(3)
Specimen Stability Information
Specimen Type Temperature Time
Varies ​ ​Refrigerate (preferred) ​7 days
​Frozen ​7 days
Rejection Criteria
Cotton or calcium alginate-tipped swab, wooden shaft swab, dry swabs, swab containing gel or charcoal
​Formalin-fixed and/or paraffin-embedded tissues
​Port-a-Cul tube
​Anaerobic fluid vials
Interference

​Interfering substances may affect the accuracy of this assay; results should always be interpreted in conjunction with clinical and epidemiological findings.

This test does not detect other Mycoplasmoides or ureaplasmas.

Useful For
Detection of Mycoplasma genitalium from genitourinary and reproductive sources.
Interpretation

A positive PCR result for the presence of a specific sequence found within the Mycoplasmoides genitalium tuf gene indicates the presence of M genitalium DNA in the specimen.

A negative PCR result indicates the absence of detectable M genitalium DNA in the specimen, it but does not rule-out infection as false-negative results may occur due to the following; inhibition of PCR, sequence variability underlying the primers or probes, or the presence of M genitalium in quantities below the limit of detection of the assay.

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Clinic Laboratories ​Monday through Friday ​3-4 days Real-Time Polymerase Chain Reaction (PCR) using LightCycler and Fluorescent Resonance Energy Transfer (FRET) 
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​87563 ​1
For most current information refer to the Marshfield Laboratory online reference manual.