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25304 Mitochondrial Antibodies (M2), IgG

Mitochondrial Antibodies (M2), IgG
Test Code: MITO
Synonyms/Keywords
Primary biliary cirrhosis, PBC, autoimmune liver disease, AMA
Useful For
Useful in the diagnosis of primary biliary cirrhosis (PBC). M2 anti-mitochondrial autoantibodies (antimitochondrial antibodies directed against the M2 autoantigen) are the most specific and sensitive immunologic markers for PBC. Definitive diagnosis of PBC should be based on the correlation of all available clinical, biochemical, radiographic, and liver biopsy information.

See the Mixed Connective Tissue Disease Panel Algorithm for suggested testing strategy. ​
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​No ​Serum ​Red Top Tube (RTT) ​Serum Separator Tube (SST) ​1.0 mL ​0.5 mL

​0.5 mL


Collection Processing Instructions

​Separate serum from cells within 2 hours of collection.

Specimen Stability Information

Specimen Type Temperature Time
​Serum ​ ​
​Refrigerate at 2-8 deg Celsius ​2 days
​Frozen at -20 deg Celsius ​> 2 days
Rejection Criteria
Gross Hemolyisis
​Gross Lipemia
​Heat treated samples
​Microbially contaminated samples
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield ​Wednesday ​1 days ​Enzyme Immunoassay (EIA)
Reference Range Information
Performing Location Reference Range
​Marshfield ​ ​ ​<= 20.0 Units Negative
​20.1-24.9 Units Equivocal
​>= 25.0 Units Positive
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​86381
Synonyms/Keywords
Primary biliary cirrhosis, PBC, autoimmune liver disease, AMA
Ordering Applications
Ordering Application Description

​Cerner
​Mitochondrial Ab (M2), IgG​
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​No ​Serum ​Red Top Tube (RTT) ​Serum Separator Tube (SST) ​1.0 mL ​0.5 mL

​0.5 mL


Collection Processing

​Separate serum from cells within 2 hours of collection.

Specimen Stability Information

Specimen Type Temperature Time
​Serum ​ ​
​Refrigerate at 2-8 deg Celsius ​2 days
​Frozen at -20 deg Celsius ​> 2 days
Rejection Criteria
Gross Hemolyisis
​Gross Lipemia
​Heat treated samples
​Microbially contaminated samples
Useful For
Useful in the diagnosis of primary biliary cirrhosis (PBC). M2 anti-mitochondrial autoantibodies (antimitochondrial antibodies directed against the M2 autoantigen) are the most specific and sensitive immunologic markers for PBC. Definitive diagnosis of PBC should be based on the correlation of all available clinical, biochemical, radiographic, and liver biopsy information.

See the Mixed Connective Tissue Disease Panel Algorithm for suggested testing strategy. ​
Reference Range Information
Performing Location Reference Range
​Marshfield ​ ​ ​<= 20.0 Units Negative
​20.1-24.9 Units Equivocal
​>= 25.0 Units Positive
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Marshfield ​Wednesday ​1 days ​Enzyme Immunoassay (EIA)
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​86381
For most current information refer to the Marshfield Laboratory online reference manual.