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25082 PCA3 (FPPCA)

PCA3 (FPPCA)
Test Code: PCA3SO
Synonyms/Keywords
Differential Display Code 3 (DD3), PCA3 (Prostate Cancer Antigen 3), PCA3, PCA-3, Prostate Cancer Gene 3
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Urine​ ​PROGENSA Urine Specimen Transport Tubes (two) 20-30 mL​ 5 mL​
Collection Processing Instructions
Collect specimen using PROGENSA Urine Specimen Transport Tube as follows:
1. Perform an attentive digital rectal exam (DRE) immediately prior to specimen collection (specimen should be collected within approximately 1 hour of DRE).
2. Patient should collect the first 20-30 mL voided urine following the DRE.
3. Process specimen within 4 hours of collection (if specimen cannot be processed within 15 minutes, store refrigerated or on ice and process within 4 hours).
    a. Invert specimen cup 5 times to re-suspend cells
    b. Add 2.5 mL of urine to each of the two GEN-PROBE PROGENSA PSA3 Urine Specimen Transport Tubes. (Do NOT puncture the foil seal on the cape 
        the transport tube; the specimen must fall between the two black fill lines on the transport tube.
    c. Tightly re-cap each urine transport tube and gently invert 5 times to mix; do not shake or vortex.
4. Send both GEN-PROBE PROGENSA transport tubes frozen.
Specimen Stability Information
Specimen Type Temperature Time​Special Container
Urine​ (PROGENSA VIAL) ​ ​ ​Ambient ​24 hours​PROGENSA VIAL
​Frozen (Preferred) ​90 days​PROGENSA VIAL
​Refrigerated ​5 days​PROGENSA VIAL
Rejection Criteria
 
Rejection Criteria
​Volume <2 mL
​Aptima media
​Hemolysis
​Incorrect collection kit
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Clinic Laboratories who forwards
to University of Michigan (MLabs)
​Monday, Wednesday, Friday ​4-9 days
Transcription Mediated Amplification (TMA)
Hybrid Protection Assay (HPA)​
Reference Lab
Test Information

Prostate cancer antigen 3 (PCA3, also known as Differential Display Code 3 or DD3) is a prostate-specific gene that was present in 95% of prostate cancer samples initially studied, and significantly over-expressed in cancer versus benign tissue. PCA3 is known to be a non-coding messenger ribonucleic acid (mRNA) with no resultant protein. Clinically, PCA3 mRNA is detectable in the urine and appears to be independent of prostate volume and serum PSA. The PCA3 urinary assay is reported out as a ratio of PCA3 mRNA to PSA mRNA.

Reference Range Information
​A score less than 25 is considered negative.
A scoree greater than or equal to 25 is considered positive.
Interpretation
​Interpretive report
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​81313
Synonyms/Keywords
Differential Display Code 3 (DD3), PCA3 (Prostate Cancer Antigen 3), PCA3, PCA-3, Prostate Cancer Gene 3
Ordering Applications
Ordering Application Description
​Centricity ​Prostate Cancer Gene 3 (FPCA3)
​Cerner ​None
​Clinical Order Manager ​Prostate Cancer Gene 3
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Urine​ ​PROGENSA Urine Specimen Transport Tubes (two) 20-30 mL​ 5 mL​
Collection Processing
Collect specimen using PROGENSA Urine Specimen Transport Tube as follows:
1. Perform an attentive digital rectal exam (DRE) immediately prior to specimen collection (specimen should be collected within approximately 1 hour of DRE).
2. Patient should collect the first 20-30 mL voided urine following the DRE.
3. Process specimen within 4 hours of collection (if specimen cannot be processed within 15 minutes, store refrigerated or on ice and process within 4 hours).
    a. Invert specimen cup 5 times to re-suspend cells
    b. Add 2.5 mL of urine to each of the two GEN-PROBE PROGENSA PSA3 Urine Specimen Transport Tubes. (Do NOT puncture the foil seal on the cape 
        the transport tube; the specimen must fall between the two black fill lines on the transport tube.
    c. Tightly re-cap each urine transport tube and gently invert 5 times to mix; do not shake or vortex.
4. Send both GEN-PROBE PROGENSA transport tubes frozen.
Specimen Stability Information
Specimen Type Temperature Time​Special Container
Urine​ (PROGENSA VIAL) ​ ​ ​Ambient ​24 hours​PROGENSA VIAL
​Frozen (Preferred) ​90 days​PROGENSA VIAL
​Refrigerated ​5 days​PROGENSA VIAL
Rejection Criteria
 
Rejection Criteria
​Volume <2 mL
​Aptima media
​Hemolysis
​Incorrect collection kit
Reference Range Information
​A score less than 25 is considered negative.
A scoree greater than or equal to 25 is considered positive.
Interpretation
​Interpretive report
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Clinic Laboratories who forwards
to University of Michigan (MLabs)
​Monday, Wednesday, Friday ​4-9 days
Transcription Mediated Amplification (TMA)
Hybrid Protection Assay (HPA)​
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​81313
For most current information refer to the Marshfield Laboratory online reference manual.