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25055 M. pneumoniae Ab, IgG and IgM (MYCO)

M. pneumoniae Ab, IgG and IgM (MYCO)
Test Code: MPNABSO
Synonyms/Keywords
M. pneumoniae, Mycoplasma Serology
Useful For

​Screening for recent or past exposure to Mycoplasma pneumoniae

This test should not be used as a screening procedure for the general population

Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Serum​ ​Red Top Tube (RTT) ​Serum Separator Tube (SST) ​1 mL ​0.5 mL
Collection Processing Instructions
​Submit specimen in plastic vial
Specimen Stability Information
Specimen Type Temperature Time
Serum​ ​ ​Refrigerated (preferred) ​14 days
​Frozen ​14 days
Rejection Criteria
Hemolysis Gross
​Lipemia Gross​
​Heat inactivated specimen ​
Interference

​A diagnosis of Mycoplasma pneumoniae infection should not be solely based on results of serologic testing for this agent. Test results should be interpreted in conjunction with clinical evaluation and the results of other diagnostic procedures (eg, molecular detection).

The continued presence or absence of antibodies cannot be used to determine the success or failure of therapy.

Testing should not be performed as a screening procedure for the general population. Testing should only be done when clinical evidence suggests the diagnosis of M pneumoniae-associated disease.

The performance of this test has not been established on neonates and immunocompromised patients.

Performance of the IgM assay has not been tested with specimens known to be positive for antibodies to organisms that are known to be associated with lower respiratory illness (ie, influenza A and B, cytomegalovirus, Chlamydophila pneumoniae, parainfluenza), and closely related serovars known to cross-react with M pneumoniae, such as Mycoplasma genitalium and Mycoplasma hominis, as well as various Ureaplasma species. Cross-reactivity studies with such organisms have not been performed with this assay.

The IgG removal system included with the IgM test system has been shown to functionally remove the IgG from specimens containing total IgG levels ranging from 300 to 600 mg/mL. The effectiveness of this removal system at IgG levels exceeding 600 mg/mL has not been established.

Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Clinic Laboratories Monday through Friday​ ​1-3 days
Enzyme Immunoassay (EIA) and
Indirect Immunofluorescence Assay (IFA)
Reference Lab
Test Information

Mycoplasma pneumoniae is a small bacterium transmitted via organism-containing droplets. It is a cause of upper respiratory infection, pharyngitis, and tracheobronchitis, particularly in children, and has been associated with approximately 20% of cases of community-acquired pneumonia. Central nervous system and cardiac manifestations are probably the most frequent extrapulmonary complications of infections due to M pneumoniae. The disease is usually self-limited, although severe disease has been reported in immunocompromised patients.

Identification of M pneumoniae by culture-based methods is time consuming and insensitive. Serology-based assays for M pneumoniae have several drawbacks. The development of IgM antibodies takes approximately 1 week and the IgM response in adults may be variable or it may be decreased in immunosuppressed individuals. Confirmation of the disease is dependent on the observation of a 4-fold rise in IgG antibody titers between acute and convalescent specimens, several weeks following the initial onset of illness, providing clinical utility only for retrospective testing. Real-time PCR offers a rapid and sensitive option for detection of M pneumoniae DNA from clinical specimens allows for diagnosis of acute or current infection.

Reference Range Information
Performing Location Reference Range
​Mayo Clinic Laboratories

​IgG: Negative

IgM: Negative

IgM by IFA: Negative

Interpretation
​IgG ELISA Result​IgM ELISA Result​Interpretation
​Positive​Negative​Results suggest past exposure
​Positive​Reactive/Equivocal​Prior exposure to Mycoplasma pneumoniae detected. Confirmatory testing for IgM to M pneumonia will be performed by an immunofluorescence assay.
​Negative​Negative​No antibodies to M pneumoniae detected. Acute infection cannot be ruled out as antibody levels may be below the limit of detection.  If clinically indicated, a second serum should be submitted in 14 to 21 days.
​NegativeReactive/Equivocal​No prior exposure to Mycoplasma pneumoniae. Confirmatory testing for IgM to M pneumonia will be performed by an immunofluorescence assay.
EquivocalNegative​Recommend follow-up testing in 10 to 14 days if clinically indicated.
​Equivocal​Reactive/Equivocal​Confirmatory testing for IgM to M pneumonia will be performed by an immunofluorescence assay.

 

Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​86738 ​2 ​Mycoplasma pneumoniae by EIA
​86738 Mycoplasma pneumoniae by indirect IFA (if needed)​
Synonyms/Keywords
M. pneumoniae, Mycoplasma Serology
Ordering Applications
Ordering Application Description
​Centricity ​M. Pneumoniae Ab (MYCPN)
​Cerner ​M. Pneumoniae Ab (85107)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Serum​ ​Red Top Tube (RTT) ​Serum Separator Tube (SST) ​1 mL ​0.5 mL
Collection Processing
​Submit specimen in plastic vial
Specimen Stability Information
Specimen Type Temperature Time
Serum​ ​ ​Refrigerated (preferred) ​14 days
​Frozen ​14 days
Rejection Criteria
Hemolysis Gross
​Lipemia Gross​
​Heat inactivated specimen ​
Interference

​A diagnosis of Mycoplasma pneumoniae infection should not be solely based on results of serologic testing for this agent. Test results should be interpreted in conjunction with clinical evaluation and the results of other diagnostic procedures (eg, molecular detection).

The continued presence or absence of antibodies cannot be used to determine the success or failure of therapy.

Testing should not be performed as a screening procedure for the general population. Testing should only be done when clinical evidence suggests the diagnosis of M pneumoniae-associated disease.

The performance of this test has not been established on neonates and immunocompromised patients.

Performance of the IgM assay has not been tested with specimens known to be positive for antibodies to organisms that are known to be associated with lower respiratory illness (ie, influenza A and B, cytomegalovirus, Chlamydophila pneumoniae, parainfluenza), and closely related serovars known to cross-react with M pneumoniae, such as Mycoplasma genitalium and Mycoplasma hominis, as well as various Ureaplasma species. Cross-reactivity studies with such organisms have not been performed with this assay.

The IgG removal system included with the IgM test system has been shown to functionally remove the IgG from specimens containing total IgG levels ranging from 300 to 600 mg/mL. The effectiveness of this removal system at IgG levels exceeding 600 mg/mL has not been established.

Useful For

​Screening for recent or past exposure to Mycoplasma pneumoniae

This test should not be used as a screening procedure for the general population

Reference Range Information
Performing Location Reference Range
​Mayo Clinic Laboratories

​IgG: Negative

IgM: Negative

IgM by IFA: Negative

Interpretation
​IgG ELISA Result​IgM ELISA Result​Interpretation
​Positive​Negative​Results suggest past exposure
​Positive​Reactive/Equivocal​Prior exposure to Mycoplasma pneumoniae detected. Confirmatory testing for IgM to M pneumonia will be performed by an immunofluorescence assay.
​Negative​Negative​No antibodies to M pneumoniae detected. Acute infection cannot be ruled out as antibody levels may be below the limit of detection.  If clinically indicated, a second serum should be submitted in 14 to 21 days.
​NegativeReactive/Equivocal​No prior exposure to Mycoplasma pneumoniae. Confirmatory testing for IgM to M pneumonia will be performed by an immunofluorescence assay.
EquivocalNegative​Recommend follow-up testing in 10 to 14 days if clinically indicated.
​Equivocal​Reactive/Equivocal​Confirmatory testing for IgM to M pneumonia will be performed by an immunofluorescence assay.

 

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
​Mayo Clinic Laboratories Monday through Friday​ ​1-3 days
Enzyme Immunoassay (EIA) and
Indirect Immunofluorescence Assay (IFA)
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​86738 ​2 ​Mycoplasma pneumoniae by EIA
​86738 Mycoplasma pneumoniae by indirect IFA (if needed)​
For most current information refer to the Marshfield Laboratory online reference manual.