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24943 Shiga Toxin, EIA (52068)

Shiga Toxin, EIA (52068)
Test Code: STECSO
Synonyms/Keywords
​Enteropathogenic E. coli
Useful For
​Enterohemorrhagic E. coli (EHEC) have been isolated from patients with hemorrhagic colitis and hemolytic-uremic syndrome, and can produce bloody diarrhea secondary to toxin damage of vascular endothelial cells. A large portion of EHEC isolates belong to the serotype 0157:H7, although at least 50 other EHEC serotypes have been reported. All EHEC strains produce potent cytotoxins called Shiga-like toxins or verotoxins, which are biochemically similar to the Shiga toxin produced by strains of Shigella dysenteriae serotype 1. This very sensitive assay detects the presence of Shiga-like Toxins 1 and 2 directly in stool specimens or culture systems.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Stool​ Cary-Blair transport medium​ 1 gram​
​Broth culture 1 culture​
​Culture isolate 1 culture​
Specimen Stability Information
Specimen Type Temperature Time
Stool/Broth cultures​ ​Refrigerate < 3 days​
Frozen at -70° C​ > 3 days​
Culture isolate​ Room temperature ​ ​
​Refrigerate ​
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Focus Diagnostics 1 to 4 days​ EIA (Enzyme Immunoassay)​
Reference Lab
Reference Range Information
Negative
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​87427
Synonyms/Keywords
​Enteropathogenic E. coli
Ordering Applications
Ordering Application Description
​Centricity ​Shiga Toxin EIA Feces (52068)
​Cerner Shiga Toxin EIA, Feces (52068)​
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Stool​ Cary-Blair transport medium​ 1 gram​
​Broth culture 1 culture​
​Culture isolate 1 culture​
Specimen Stability Information
Specimen Type Temperature Time
Stool/Broth cultures​ ​Refrigerate < 3 days​
Frozen at -70° C​ > 3 days​
Culture isolate​ Room temperature ​ ​
​Refrigerate ​
Useful For
​Enterohemorrhagic E. coli (EHEC) have been isolated from patients with hemorrhagic colitis and hemolytic-uremic syndrome, and can produce bloody diarrhea secondary to toxin damage of vascular endothelial cells. A large portion of EHEC isolates belong to the serotype 0157:H7, although at least 50 other EHEC serotypes have been reported. All EHEC strains produce potent cytotoxins called Shiga-like toxins or verotoxins, which are biochemically similar to the Shiga toxin produced by strains of Shigella dysenteriae serotype 1. This very sensitive assay detects the presence of Shiga-like Toxins 1 and 2 directly in stool specimens or culture systems.
Reference Range Information
Negative
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Focus Diagnostics 1 to 4 days​ EIA (Enzyme Immunoassay)​
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​87427
For most current information refer to the Marshfield Laboratory online reference manual.