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23196 Estriol, Unconjugated, Serum (UE3)

Estriol, Unconjugated, Serum (UE3)
Test Code: MISC
Synonyms/Keywords
​E3 (Estriol), Estriol, UE3 (Unconjugated Estriol), Unconjugated Estriol
Useful For

A part of second trimester or cross-trimester biochemical screening for Down syndrome and trisomy 18 syndrome

A marker of fetal demise

An adjunct biomarker in the prenatal diagnosis of disorders of fetal steroid metabolism, including Smith-Lemli-Opitz syndrome (SLO)(3-4), and X-linked ichthyosis (placental sulfatase deficiency disorders)

Evaluating primary or secondary fetal adrenal insufficiency after excluding other rare single gene defects, including aromatase deficiency, 17 alpha-hydroxylase deficiency and/or various forms of congenital adrenal hyperplasia

Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​Serum Red Top Tube (RTT)​ Serum Separator Tube (SST)​ 0.6 mL​ 0.5 mL​
Specimen Stability Information
Specimen Type Temperature Time
Serum​ ​ Refrigerated (preferred)​ 14 days​
Frozen ​ 90 days​
Rejection Criteria
Gross hemolysis
Interference

​Like any immunoassay, this test can occasionally be subject to analytical interferences. Some patients who have been exposed to animal antigens, either in the environment or as part of treatment or imaging procedures, may have circulating anti-animal antibodies present. These antibodies may interfere with the assay reagents to produce unreliable results. If the clinical picture is inconsistent with the test results, clinicians should consider the possibility of a preanalytical or analytical error and contact the laboratory.

Performing Laboratory Information
Performing Location Day(s) Test Performed Report Available Methodology/Instrumentation
Mayo Clinic Laboratories
Monday through Saturday​
1 to 3 days
Immunoenzymatic Assay​
Reference Lab
Reference Range Information
Males: <0.07 ng/mL
Females: < 0.08 ng/mL
Interpretation

In second trimester maternal serum screening (QUAD), , unconjugated E3 (uE3) forms part of a complex, multivariate risk calculation formula, using maternal age, gestational stage, and other demographic information, in addition to the results of the biochemical markers, for Down syndrome and trisomy 18 risk calculation.

A serum uE3 <0.15 multiples of the gestational age median in women, who otherwise screen negative in the quad test, can indicate Smith-Lemli-Opitz syndrome and X-linked ichthyosis.

A low uE3 level can indicate the possibility of aromatase deficiency, congenital adrenal hyperplasia, primary or secondary (including maternal corticosteroid therapy) fetal adrenal insufficiency and/or fetal demise.

Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​82677
Synonyms/Keywords
​E3 (Estriol), Estriol, UE3 (Unconjugated Estriol), Unconjugated Estriol
Ordering Applications
Ordering Application Description
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​Serum Red Top Tube (RTT)​ Serum Separator Tube (SST)​ 0.6 mL​ 0.5 mL​
Specimen Stability Information
Specimen Type Temperature Time
Serum​ ​ Refrigerated (preferred)​ 14 days​
Frozen ​ 90 days​
Rejection Criteria
Gross hemolysis
Interference

​Like any immunoassay, this test can occasionally be subject to analytical interferences. Some patients who have been exposed to animal antigens, either in the environment or as part of treatment or imaging procedures, may have circulating anti-animal antibodies present. These antibodies may interfere with the assay reagents to produce unreliable results. If the clinical picture is inconsistent with the test results, clinicians should consider the possibility of a preanalytical or analytical error and contact the laboratory.

Useful For

A part of second trimester or cross-trimester biochemical screening for Down syndrome and trisomy 18 syndrome

A marker of fetal demise

An adjunct biomarker in the prenatal diagnosis of disorders of fetal steroid metabolism, including Smith-Lemli-Opitz syndrome (SLO)(3-4), and X-linked ichthyosis (placental sulfatase deficiency disorders)

Evaluating primary or secondary fetal adrenal insufficiency after excluding other rare single gene defects, including aromatase deficiency, 17 alpha-hydroxylase deficiency and/or various forms of congenital adrenal hyperplasia

Reference Range Information
Males: <0.07 ng/mL
Females: < 0.08 ng/mL
Interpretation

In second trimester maternal serum screening (QUAD), , unconjugated E3 (uE3) forms part of a complex, multivariate risk calculation formula, using maternal age, gestational stage, and other demographic information, in addition to the results of the biochemical markers, for Down syndrome and trisomy 18 risk calculation.

A serum uE3 <0.15 multiples of the gestational age median in women, who otherwise screen negative in the quad test, can indicate Smith-Lemli-Opitz syndrome and X-linked ichthyosis.

A low uE3 level can indicate the possibility of aromatase deficiency, congenital adrenal hyperplasia, primary or secondary (including maternal corticosteroid therapy) fetal adrenal insufficiency and/or fetal demise.

For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Report Available Methodology/Instrumentation
Mayo Clinic Laboratories
Monday through Saturday​
1 to 3 days
Immunoenzymatic Assay​
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​82677
For most current information refer to the Marshfield Laboratory online reference manual.