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23188 First Trimester Screen

First Trimester Screen
Test Code: FTSNTSO
Synonyms/Keywords
​NTD First Trimester
Test Components

An ultrasound examination is needed to confirm gestational age, measure the nuchal translucency (NT), and assess the fetal nasal bone.

A maternal blood sample is analyzed for three biochemical markers: alpha fetoprotein (AFP), free beta human chorionic gonadotropin (free beta hCG) and pregnancy associated plasma protein-A (PAPP-A)

Useful For
​This test is a prenatal screen for specific risk for Down Syndrome, Trisomy-18 (Edwards Syndrome), and Trisomy- 13 (Patau Syndrome).
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​Whole Blood Fingerstick from mother utilizing filter card provided by NTD Laboratories ​5 spots ​2 spots
Collection Processing Instructions

Fingerstick from mother utilizing filter card provided by NTD Laboratories.  Phone number for supplies is 888-683-5227. 

A patient ultra sound is NOT required for the first trimester screen to be completed.

Gestational Age: Draw blood specimen between 9 weeks 0 days and 13 weeks 6 days gestation. Nuchal

translucency and nasal bone should be performed between 11 weeks 1 day and 13 weeks 6 days gestation(CRL:

45-84mm).

Specimen Stability Information
Specimen Type Temperature​Time
​Whole blood on filter card ​Ambient​30 days
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
NTD Laboratories​ ​Monday through Friday ​3 days ​Chemiluminescent Immunoassay
Reference Lab
Test Information

A first trimester screen for Down syndrome and trisomy 18/13 which analyzes free Beta HCG (fbhCG), pregnancy-associated plasma protein A (PAPP-A), alphafetoprotein (AFP), nuchal translucency measurement, and nasal bone assessment. Analyte values are compared to median values at a given gestational age and multiple of the median (MoM) results are obtained. The MoM results are used in a multivariate algorithm that includes the mother's age to report a risk assessment for Down syndrome and trisomy 18/13.

Reference Range Information
Performing Location Reference Range
​NTD Laboratories ​Interpretive Report
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​82105 ​1 Alpha-fetoprotein, sera
​84163 ​1 ​Pregnancy-associated plasma protein (PAPP-A)
​84704 ​1 ​hCG, free beta chain
Synonyms/Keywords
​NTD First Trimester
Test Components

An ultrasound examination is needed to confirm gestational age, measure the nuchal translucency (NT), and assess the fetal nasal bone.

A maternal blood sample is analyzed for three biochemical markers: alpha fetoprotein (AFP), free beta human chorionic gonadotropin (free beta hCG) and pregnancy associated plasma protein-A (PAPP-A)

Ordering Applications
Ordering Application Description
​Centricity ​First Trimester Screen with NTP
​Cerner ​First Trimester Screen
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
​Whole Blood Fingerstick from mother utilizing filter card provided by NTD Laboratories ​5 spots ​2 spots
Collection Processing

Fingerstick from mother utilizing filter card provided by NTD Laboratories.  Phone number for supplies is 888-683-5227. 

A patient ultra sound is NOT required for the first trimester screen to be completed.

Gestational Age: Draw blood specimen between 9 weeks 0 days and 13 weeks 6 days gestation. Nuchal

translucency and nasal bone should be performed between 11 weeks 1 day and 13 weeks 6 days gestation(CRL:

45-84mm).

Specimen Stability Information
Specimen Type Temperature​Time
​Whole blood on filter card ​Ambient​30 days
Useful For
​This test is a prenatal screen for specific risk for Down Syndrome, Trisomy-18 (Edwards Syndrome), and Trisomy- 13 (Patau Syndrome).
Test Components

An ultrasound examination is needed to confirm gestational age, measure the nuchal translucency (NT), and assess the fetal nasal bone.

A maternal blood sample is analyzed for three biochemical markers: alpha fetoprotein (AFP), free beta human chorionic gonadotropin (free beta hCG) and pregnancy associated plasma protein-A (PAPP-A)

Reference Range Information
Performing Location Reference Range
​NTD Laboratories ​Interpretive Report
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
NTD Laboratories​ ​Monday through Friday ​3 days ​Chemiluminescent Immunoassay
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​82105 ​1 Alpha-fetoprotein, sera
​84163 ​1 ​Pregnancy-associated plasma protein (PAPP-A)
​84704 ​1 ​hCG, free beta chain
For most current information refer to the Marshfield Laboratory online reference manual.