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# A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
Culture, Group B Strep, Female
Test Code: GBS
Synonyms/Keywords
GBS Screen
Useful For
​Screening of Group B streptococci carriage in pregnant females.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Vaginal introitus and/or anorectum​

ESwab

​Other Amies medium based swab 1 swab ​1 swab ​1 swab
Collection Processing Instructions

A specimen collected with a speculum is not recommended.

Swabs:

- ESwab: collect one swab for Gram stain, aerobic and anaerobic cultures, and an additional swab for each additional culture type or stain (e.g. fungus or mycobacteria).

- Other Amies medium swab types: collect one swab for every culture and stain (including Gram) to be ordered.

See eSwab collection video for more information

Specimens should be delivered to the microbiology laboratory as soon as possible.  Culture of specimens older than 24 hours may lead to a reduction in recovery of microorganisms.

For more information on Specimen Collection Media/Swab, see Specimen Transport Pictorial.

Specimen Stability Information
Specimen Type Temperature Time
​Swabs ​ Room Temperature​ <= 4 hours​
​Refrigerate ​> 4 hours
Rejection Criteria
Cervical, perianal, perirectal and perineal swabs
Dry swabs​
​Leaking specimens
​Specimens older than 4 days
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Diagnostic Treatment Center​ ​Monday through Sunday ​3 days​ ​Conventional culture, standard susceptibility, manual or automated identification methods.
​Marshfield Monday through Sunday 3 days​ LIM broth culture​, manual or automated identification methods, and nucleic acid sequence analysis as needed.
Test Information
​The Center for Disease Control, in conjunction with The American College of Obstetricians and Gynecologists and the American Academy of Pediatrics, have published guidelines for optimal practices for screening for Group B streptococcal (GBS) carriers.  These guidelines recommend universal prenatal screening for vaginal and rectal GBS colonization of all pregnant women at 35-37 weeks gestation and/or women of child-bearing age (14-45).
 
Additionally, this culture incorporates the following from the 2010 CDC guidelines:
-  Use of LIM selective enrichment broth;
-  Use of an FDA-cleared D-Test to detect inducible clindamycin resistance.
-  Reporting all culture isolates exhibiting inducible clindamycin resistance as being resistant to clindamycin.
 
(Centers for Disease Control and Prevention, Prevention of Perinatal Group B Streptococcal Disease, Revised Guidelines from CDC, 2010.  MMWR 2010, 59 [RR-10].)
 
‘ESwab’ is a trademark of Copan Diagnostics.
Reference Range Information
Performing Location Reference Range
All Performing Sites No Group B Strep isolated​
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
87081​ Culture​
87147​ Immunologic method, per antiserum​ as needed​
87077​ Aerobic isolate, definitive ID as needed​
​87153 ​Identification by nucleic acid sequencing method ​as needed
Synonyms/Keywords
GBS Screen
Ordering Applications
Ordering Application Description
​Centricity ​Cult. Group B Strep, Female
​Cerner Culture, Group B Strep, Female​
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Vaginal introitus and/or anorectum​

ESwab

​Other Amies medium based swab 1 swab ​1 swab ​1 swab
Collection Processing Instructions

A specimen collected with a speculum is not recommended.

Swabs:

- ESwab: collect one swab for Gram stain, aerobic and anaerobic cultures, and an additional swab for each additional culture type or stain (e.g. fungus or mycobacteria).

- Other Amies medium swab types: collect one swab for every culture and stain (including Gram) to be ordered.

See eSwab collection video for more information

Specimens should be delivered to the microbiology laboratory as soon as possible.  Culture of specimens older than 24 hours may lead to a reduction in recovery of microorganisms.

For more information on Specimen Collection Media/Swab, see Specimen Transport Pictorial.

Specimen Stability Information
Specimen Type Temperature Time
​Swabs ​ Room Temperature​ <= 4 hours​
​Refrigerate ​> 4 hours
Rejection Criteria
Cervical, perianal, perirectal and perineal swabs
Dry swabs​
​Leaking specimens
​Specimens older than 4 days
Useful For
​Screening of Group B streptococci carriage in pregnant females.
Test Information
​The Center for Disease Control, in conjunction with The American College of Obstetricians and Gynecologists and the American Academy of Pediatrics, have published guidelines for optimal practices for screening for Group B streptococcal (GBS) carriers.  These guidelines recommend universal prenatal screening for vaginal and rectal GBS colonization of all pregnant women at 35-37 weeks gestation and/or women of child-bearing age (14-45).
 
Additionally, this culture incorporates the following from the 2010 CDC guidelines:
-  Use of LIM selective enrichment broth;
-  Use of an FDA-cleared D-Test to detect inducible clindamycin resistance.
-  Reporting all culture isolates exhibiting inducible clindamycin resistance as being resistant to clindamycin.
 
(Centers for Disease Control and Prevention, Prevention of Perinatal Group B Streptococcal Disease, Revised Guidelines from CDC, 2010.  MMWR 2010, 59 [RR-10].)
 
‘ESwab’ is a trademark of Copan Diagnostics.
Reference Range Information
Performing Location Reference Range
All Performing Sites No Group B Strep isolated​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Diagnostic Treatment Center​ ​Monday through Sunday ​3 days​ ​Conventional culture, standard susceptibility, manual or automated identification methods.
​Marshfield Monday through Sunday 3 days​ LIM broth culture​, manual or automated identification methods, and nucleic acid sequence analysis as needed.
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
87081​ Culture​
87147​ Immunologic method, per antiserum​ as needed​
87077​ Aerobic isolate, definitive ID as needed​
​87153 ​Identification by nucleic acid sequencing method ​as needed
For most current information refer to the Marshfield Laboratory online reference manual.