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# A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
Streptococcal Antibodies (Anti-DNase B and Anti-Streptolysin O)
Test Code: ASA
Synonyms/Keywords
Streptococcal Antibodies (ADNB/ASO), Streptococcal Abs, anti-deoxyribonuclease-B (ADNase), ADNB; ASO, Antistreptolysin O (ASLO) ​
Test Components
Anti-DNAse B and Anti-Streptolysin O​
Useful For
Testing for ADNase-B antibodies is important for confirming an existing or recent streptococcal infection.  The antibody response to streptococcal ADNase-B is stronger, but begins later than the formation of ASLO.  ADNB documents recent Group A Streptococcus infection and is the most consistent of available tests. ​
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
No​ Serum​
Red Top Tube
(RTT)​
Serum Separator Tube (SST)​ 0.5 mL 0.4 mL 0.3 mL
Specimen Stability Information
Specimen Type Temperature Time
Serum​ Refrigerate​ 7 days​
Frozen 3 months​
Rejection Criteria
Plasma samples
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday​ 6-8 hours​ Nephelometry/Siemens BN ProSpec​
Reference Range Information
Performing Location Reference Range
Marshfield​ ​
Reference range for ASLO:
 
< or = 5 years old:   < or = 85 U/mL
Ages 5-18:   < or = 170 U/mL
Adult (>18):   < or = 85 U/mL​
Reference range for ADNase B:
 
< or = 5 years old:  < or = 250 U/mL
Ages 5-18:   < or = 375 U/mL
Adult (>18):   < or = 300 U/mL​
Interpretation
Group A streptococci account for the majority of stretococcal infections (rheumatic fever, scarlet fever, tonsillitis and glomerulonephritis) in humans. An infection of Group A Strep causes the body to produce antibodies that attack the body's own tissues.  The sensitivity of each test for streptococcal infection is approximately 80-85%. Because some patients will be positive for one antibody and not the other, determining both ASO and ADNB levels increases the sensitivity to 95%. Antibody response to streptolysin O is weak in the case of skin infections, whereas the Anti-DNAse B titer will increase.  The antibody response to streptococcal ADNase B is stronger, but begins later than the response to ASO.​
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
86215​ ADNase B​
86060​ Anti-Streptolysin O​
Synonyms/Keywords
Streptococcal Antibodies (ADNB/ASO), Streptococcal Abs, anti-deoxyribonuclease-B (ADNase), ADNB; ASO, Antistreptolysin O (ASLO) ​
Test Components
Anti-DNAse B and Anti-Streptolysin O​
Ordering Applications
Ordering Application Description
​Centricity ​Strep Trep Ab.-ASO/ADNB
​Cerner Antistreptolysin O Titer​
​COM ​Streptococcal Abs-ASO/ADNB
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Fasting Required Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
No​ Serum​
Red Top Tube
(RTT)​
Serum Separator Tube (SST)​ 0.5 mL 0.4 mL 0.3 mL
Specimen Stability Information
Specimen Type Temperature Time
Serum​ Refrigerate​ 7 days​
Frozen 3 months​
Rejection Criteria
Plasma samples
Useful For
Testing for ADNase-B antibodies is important for confirming an existing or recent streptococcal infection.  The antibody response to streptococcal ADNase-B is stronger, but begins later than the formation of ASLO.  ADNB documents recent Group A Streptococcus infection and is the most consistent of available tests. ​
Reference Range Information
Performing Location Reference Range
Marshfield​ ​
Reference range for ASLO:
 
< or = 5 years old:   < or = 85 U/mL
Ages 5-18:   < or = 170 U/mL
Adult (>18):   < or = 85 U/mL​
Reference range for ADNase B:
 
< or = 5 years old:  < or = 250 U/mL
Ages 5-18:   < or = 375 U/mL
Adult (>18):   < or = 300 U/mL​
Interpretation
Group A streptococci account for the majority of stretococcal infections (rheumatic fever, scarlet fever, tonsillitis and glomerulonephritis) in humans. An infection of Group A Strep causes the body to produce antibodies that attack the body's own tissues.  The sensitivity of each test for streptococcal infection is approximately 80-85%. Because some patients will be positive for one antibody and not the other, determining both ASO and ADNB levels increases the sensitivity to 95%. Antibody response to streptolysin O is weak in the case of skin infections, whereas the Anti-DNAse B titer will increase.  The antibody response to streptococcal ADNase B is stronger, but begins later than the response to ASO.​
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Marshfield​ Monday through Friday​ 6-8 hours​ Nephelometry/Siemens BN ProSpec​
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
86215​ ADNase B​
86060​ Anti-Streptolysin O​
For most current information refer to the Marshfield Laboratory online reference manual.