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# A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
Hgb Electrophoresis Cascade (HBELC)
Test Code: HBESO
Synonyms/Keywords
​Lab Ref Code:  81626 , A2 Hemoglobin, Alpha Globin Variant, Barts Hemoglobin, Beta Globin Variant, Beta-Thalassemia, Fetal Hemoglobin, fetaldex (Hemoglobin F), Hemoglobin A2, Hemoglobin Cascade, Hemoglobin Electrophoresis Cascade Level 1, Hemoglobin F, Hemoglobin Mass Spec Studies, Hemoglobin Molecular studies, Hemoglobin S quant, Hemoglobin Variant, Hemoglobin-H Disease, HGB (Hemoglobin) Electrophoresis, Isoelectric Focusing, Sickling Test
Useful For
Diagnosis of thalassemias and hemoglobin variants
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Whole Blood​ ​EDTA Lavender Top Tube (LTT) 6 mL​ ​1 mL
Collection Processing Instructions
Collection Instructions: Do not transfer blood to other containers.
Additional Information:
1. Patient's age is required.
2. Include recent transfusion information.
3. For information on thalassemias and appropriate test ordering, see Thalassemia Tests in Special Instructions.
 
Forms:
Specimen Stability Information
Specimen Type Temperature Time
​Whole Blood ​Refrigerated ​10 days
Rejection Criteria
Hemolysis No (lab will evaluate)
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Mayo Medical Laboratories​​
Monday through Saturday 
1 day/2-25 days if structural and/or molecular studies are required​
A2F/83341: Cation Exchange/High-Performance Liquid Chromatography (HPLC)
HBEL/81428: Capillary Electrophoresis
SDEX/9180: Hemoglobin S Solubility
UNHB/9095: Isopropanol Stability
IEF/81644: Isoelectric Focusing
HPFH/8270: Flow Cytometry
MASS/60286: Mass Spectrometry (MS)
HGBMO/29374: Polymerase Chain Reaction (PCR) Analysis/Multiplex Ligation-Dependent Probe Amplification (MLPA), Polymerase Chain Reaction (PCR)/DNA Sequencing
(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)
Reference Lab
Test Information
Hemoglobin electrophoresis cascade will always include hemoglobin A(2) and F and hemoglobin electrophoresis.
 
Reflex testing-Hemoglobin electrophoresis reflex testing performed at additional charge, may include any or all of the following as indicated to identify rare hemoglobin variants present: hemoglobin S screen, unstable hemoglobin, IEF confirms, hemoglobin variant by mass spec, hemoglobin F red cell distribution, beta-globin gene, large del/dup, alpha-globin gene sequencing, and beta-globin gene sequencing.
Reference Range Information
Reference Range
HEMOGLOBIN A
1-30 days: 5.9-77.2%
1-2 months: 7.9-92.4%
3-5 months: 54.7-97.1%
6-8 months: 80.0-98.0%
9-12 months: 86.2-98.0%
13-17 months: 88.8-98.0%
18-23 months: 90.4-98.0%
> or =24 months: 95.8-98.0%
 
HEMOGLOBIN A2
1-30 days: 0.0-2.1%
1-2 months: 0.0-2.6%
3-5 months: 1.3-3.1%
> or =6 months: 2.0-3.3%
 
HEMOGLOBIN F
1-30 days: 22.8-92.0%
1-2 months: 7.6-89.8%
3-5 months: 1.6-42.2%
6-8 months: 0.0-16.7%
9-12 months: 0.0-10.5%
13-17 months: 0.0-7.9%
18-23 months: 0.0-6.3%
> or =24 months: 0.0-0.9%
 
VARIANT
No abnormal variants
 
VARIANT 2
No abnormal variants
 
VARIANT 3
No abnormal variants
Interpretation
​The types of hemoglobin present are identified, quantitated, and an interpretive report is issued
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​83021 ​1 ​Quantitation by HPLC
​83020 ​1 ​Quantitation by electrophoresis
82664​ ​1 Electrophoresis, not elsewhere specified ​​ If Appropriate​​​
​83068 ​1 Hemoglobin, Unstable Screen​​ If Appropriate​​​
​83789 ​1 Hemoglobin Variant by Mass Spectrometry​​ If Appropriate​​​
​81259 ​1 Alpha Globin Gene Sequencing If Appropriate​​​
​81364 ​1 Beta Globin Gene Sequencing If Appropriate​​​
​81363 ​1 Beta Globin Cluster Locus Del/Dup If Appropriate​​​
​85660 ​1
Hemoglobin S, Screen
If Appropriate​​​
88184​ ​1 Hemoglobin F, Red Blood Cell Distribution​​ If Appropriate​​​
81479​ ​1 ​Gamma Globin Full Gene Sequencing ​If Appropriate​​​
​88235 ​1 ​Amniotic Fluid Culture ​​If Appropriate​​​
88240​ ​1 ​Cryopreservation ​​​If Appropriate
​88233 ​1 ​Tissue Culture Skin/Biopsy ​​​​If Appropriate
​81265 ​1 ​Comp analysis using STR ​​​​​If Appropriate
​81266 ​1 ​Additional comp analysis w/STR ​​​​​If Appropriate
Synonyms/Keywords
​Lab Ref Code:  81626 , A2 Hemoglobin, Alpha Globin Variant, Barts Hemoglobin, Beta Globin Variant, Beta-Thalassemia, Fetal Hemoglobin, fetaldex (Hemoglobin F), Hemoglobin A2, Hemoglobin Cascade, Hemoglobin Electrophoresis Cascade Level 1, Hemoglobin F, Hemoglobin Mass Spec Studies, Hemoglobin Molecular studies, Hemoglobin S quant, Hemoglobin Variant, Hemoglobin-H Disease, HGB (Hemoglobin) Electrophoresis, Isoelectric Focusing, Sickling Test
Ordering Applications
Ordering Application Description
​Centricity ​Hemoglobin Electrophoresis
​Cerner ​Hemoglobin Electrophoresis Cascade (81626)
If the ordering application you are looking for is not listed, contact your local laboratory for assistance.
Specimen Requirements
Specimen Type Preferred Container/Tube Acceptable Container/Tube Specimen Volume Specimen Minimum Volume
(allows for 1 repeat)
Pediatric Minimum Volume
(no repeat)
Whole Blood​ ​EDTA Lavender Top Tube (LTT) 6 mL​ ​1 mL
Collection Processing Instructions
Collection Instructions: Do not transfer blood to other containers.
Additional Information:
1. Patient's age is required.
2. Include recent transfusion information.
3. For information on thalassemias and appropriate test ordering, see Thalassemia Tests in Special Instructions.
 
Forms:
Specimen Stability Information
Specimen Type Temperature Time
​Whole Blood ​Refrigerated ​10 days
Rejection Criteria
Hemolysis No (lab will evaluate)
Useful For
Diagnosis of thalassemias and hemoglobin variants
Test Information
Hemoglobin electrophoresis cascade will always include hemoglobin A(2) and F and hemoglobin electrophoresis.
 
Reflex testing-Hemoglobin electrophoresis reflex testing performed at additional charge, may include any or all of the following as indicated to identify rare hemoglobin variants present: hemoglobin S screen, unstable hemoglobin, IEF confirms, hemoglobin variant by mass spec, hemoglobin F red cell distribution, beta-globin gene, large del/dup, alpha-globin gene sequencing, and beta-globin gene sequencing.
Reference Range Information
Reference Range
HEMOGLOBIN A
1-30 days: 5.9-77.2%
1-2 months: 7.9-92.4%
3-5 months: 54.7-97.1%
6-8 months: 80.0-98.0%
9-12 months: 86.2-98.0%
13-17 months: 88.8-98.0%
18-23 months: 90.4-98.0%
> or =24 months: 95.8-98.0%
 
HEMOGLOBIN A2
1-30 days: 0.0-2.1%
1-2 months: 0.0-2.6%
3-5 months: 1.3-3.1%
> or =6 months: 2.0-3.3%
 
HEMOGLOBIN F
1-30 days: 22.8-92.0%
1-2 months: 7.6-89.8%
3-5 months: 1.6-42.2%
6-8 months: 0.0-16.7%
9-12 months: 0.0-10.5%
13-17 months: 0.0-7.9%
18-23 months: 0.0-6.3%
> or =24 months: 0.0-0.9%
 
VARIANT
No abnormal variants
 
VARIANT 2
No abnormal variants
 
VARIANT 3
No abnormal variants
Interpretation
​The types of hemoglobin present are identified, quantitated, and an interpretive report is issued
For more information visit:
Performing Laboratory Information
Performing Location Day(s) Test Performed Analytical Time Methodology/Instrumentation
Mayo Medical Laboratories​​
Monday through Saturday 
1 day/2-25 days if structural and/or molecular studies are required​
A2F/83341: Cation Exchange/High-Performance Liquid Chromatography (HPLC)
HBEL/81428: Capillary Electrophoresis
SDEX/9180: Hemoglobin S Solubility
UNHB/9095: Isopropanol Stability
IEF/81644: Isoelectric Focusing
HPFH/8270: Flow Cytometry
MASS/60286: Mass Spectrometry (MS)
HGBMO/29374: Polymerase Chain Reaction (PCR) Analysis/Multiplex Ligation-Dependent Probe Amplification (MLPA), Polymerase Chain Reaction (PCR)/DNA Sequencing
(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)
Reference Lab
For billing questions, see Contacts
Outreach CPTs
CPT Modifier
(if needed)
Quantity Description Comments
​83021 ​1 ​Quantitation by HPLC
​83020 ​1 ​Quantitation by electrophoresis
82664​ ​1 Electrophoresis, not elsewhere specified ​​ If Appropriate​​​
​83068 ​1 Hemoglobin, Unstable Screen​​ If Appropriate​​​
​83789 ​1 Hemoglobin Variant by Mass Spectrometry​​ If Appropriate​​​
​81259 ​1 Alpha Globin Gene Sequencing If Appropriate​​​
​81364 ​1 Beta Globin Gene Sequencing If Appropriate​​​
​81363 ​1 Beta Globin Cluster Locus Del/Dup If Appropriate​​​
​85660 ​1
Hemoglobin S, Screen
If Appropriate​​​
88184​ ​1 Hemoglobin F, Red Blood Cell Distribution​​ If Appropriate​​​
81479​ ​1 ​Gamma Globin Full Gene Sequencing ​If Appropriate​​​
​88235 ​1 ​Amniotic Fluid Culture ​​If Appropriate​​​
88240​ ​1 ​Cryopreservation ​​​If Appropriate
​88233 ​1 ​Tissue Culture Skin/Biopsy ​​​​If Appropriate
​81265 ​1 ​Comp analysis using STR ​​​​​If Appropriate
​81266 ​1 ​Additional comp analysis w/STR ​​​​​If Appropriate
For most current information refer to the Marshfield Laboratory online reference manual.